摘要
构建人脐动脉平滑肌细胞(HUASMC)氧化应激模型,采用栝楼籽蛋白酶解物(Protease Hydrolysates of Trichosanthes Seed,PHTS)处理细胞,检测细胞存活率和总谷胱甘肽(GSH)、内皮素(ET)水平,分析过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的基因与蛋白表达。结果表明,栝楼籽蛋白酶解物可有效提升HUASMC细胞的存活率和GSH、CAT、SOD表达水平,抑制ET分泌。
An oxidative stress model of human umbilical artery smooth muscle cells HUASMC is established,and Protease Hydrolysates of Trichosanthes Seed(PHTS)are used to process cells for testing the indexes such as cell survival rate,total glutathione(GSH),endothelin(ET)levels and analyzing the gene expression,protein expression of catalase(CAT)and superoxide dismutase(SOD).The results indicate that PHTS can effectively prolong survival rate of HUASMC cells,expression level of GSH,CAT and SOD,and inhibit ET secretion.
作者
张瑾莉
徐敏
杨希
朱丽娅
袁翔宇
ZHANG Jinli;XU Min;YANG Xi;ZHU Liya;YUAN Xiangyu(Anhui Vocational College of Grain Engineering,Hefei 230011,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)
出处
《长春工业大学学报》
CAS
2022年第2期181-186,共6页
Journal of Changchun University of Technology
基金
安徽省自然科学研究项目(KJ2019A1267)
安徽省教育厅高校优秀拔尖人才培育资助项目(gxgnfx2020179)
安徽省职业与成人教育学会教育科研规划课题(2020azcg141)
安徽省高校优秀青年人才支持计划项目(gxyqZD2021165)。
关键词
栝楼籽蛋白酶解物
抗氧化作用
HUASMC细胞
血压
Protease Hydrolysates of Trichosanthes Seed(PHTS)
antioxidation
HUASMC cells
blood pressure
