通心降脂滴丸在缺氧损伤中的保护作用研究

PROTECTIVE EFFECT OF TONGXIN JIANGZHI DROPPING PILLS ON HYPOXIA INJURY

目的观察通心降脂滴丸对小鼠心肌细胞缺氧损伤的保护作用,为其临床试验及临床应用提供药理学依据。方法体内实验采用昆明种小白鼠60只,按随机数表法分为5组,每组12只,分为模型组、阳性对照组、通心降脂滴丸低、中、高剂量组,观察小鼠在缺氧状态下的平均存活时间;体外实验将小鼠心肌细胞随机分为空白对照组、缺氧模型组、阳性对照组、通心降脂滴丸低、中和高剂量组,建立缺氧损伤模型,各组取培养液上清液测定乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)的活力以及丙二醛(MDA)的含量。结果体内实验中,与模型组比较,通心降脂滴丸中、高剂量组可明显延长小鼠在缺氧状态下的存活时间,组间差异有统计学意义(P<0.05)。体外实验中与空白对照组比较,缺氧模型组LDH、CK的活力升高,SOD的活力下降,MDA含量升高,组间差异有统计学意义(P<0.05);与缺氧模型组比较,通心降脂滴丸3个剂量组的LDH活力降低,SOD活力升高,MDA含量减少,中、高剂量组CK的活性降低,组间差异有统计学意义(P<0.05)。结论通心降脂滴丸可提高小鼠的耐缺氧能力并对缺氧损伤的心肌细胞具有一定的保护作用。

Objective To observe the protective effect of Tongxin Jiangzhi dropping pill on hypoxia injury of mice and mouse cardiomyocytes,so as to provide pharmacodynamic basis for clinical trial and clinical application of Tongxin Jiangzhi dropping pills.Methods In vivo experiment,sixty Kunming mice were randomly divided into five groups with twelve mice in each group,which were model group,positive control group and low,medium and high dose groups of Tongxin Jiangzhi dropping pills.The average survival time of mice under hypoxia was observed.In vitro experiment,mouse cardiomyocytes were randomly divided into blank control group,hypoxia model group,positive control group,low,medium and high dose groups of Tongxin Jiangzhi dropping pills,and established hypoxia injury model.The supernatant of culture solution was taken from each group to determine the activity of lactate dehydrogenase(LDH),creatine kinase(CK),the superoxide dismutase(SOD)and the content of malondialdehyde(MDA).Results In vivo experiment,compared with the model group,the medium and high dose groups of Tongxin Jiangzhi dropping pills could significantly prolong the survival time of mice under hypoxia(P<0.05).In vitro experiment,compared with the blank control group,the activities of CK and LDH increased,the activity of SOD decreased and the content of MDA increased significantly(P<0.05);compared with hypoxia model group,low,medium and high dose groups of Tongxin Jiangzhi dropping pills can reduce the activity of LDH,can increase the activity of SOD and reduce the content of MDA,middle and high dose groups can reduce the activity of CK significantly(P<0.05).Conclusions Tongxin Jiangzhi dropping pills can improve the ability of hypoxia tolerance in mice and have a certain protective effect on hypoxia damaged cardiomyocytes.