灯草灸对热性惊厥幼年大鼠大脑皮质内GABA、Glu含量的影响

Effect of Lamp Grass Moxibustion on the Contents of GABA and Glutamic Acid in Cerebral Cortex of Young Rats with Febrile Convulsion

目的观察灯草灸疗法对热性惊厥幼年大鼠大脑匀浆内谷氨酸(Glu)、γ-氨基丁酸(GABA)含量的影响及其可能的作用机制。方法40只健康SD幼年大鼠,随机分为模型组、对照组、安定组、灯草灸组和安定+灯草灸组,每组8只,均制作热性惊厥大鼠模型。然后检测各组大鼠大脑皮Glu、GABA含量。结果与对照组相比,模型组大鼠大脑皮质Glu含量明显升高(P<0.01)。与模型组相比,安定组、安定+灯草灸组大鼠大脑皮质Glu含量明显降低(P<0.01),模型组与灯草灸组之间无显著性差异(P>0.05),安定组、灯草灸组、安定+灯草灸组之间无显著性差异(P>0.05),安定+灯草灸组与安定组相比有显著性差异(P<0.05)、与灯草灸组相比有极显著性差异(P<0.01)。与对照组相比,模型组大鼠大脑皮质GABA含量明显降低(P<0.01);模型组与安定组、灯草灸组、安定+灯草灸组相比有极显著性差异(P<0.01);安定组与灯草灸组相比无显著差异(P>0.05),安定组与安定+灯草灸组相比有显著差异(P<0.05),灯草灸组与安定+灯草灸组相比有显著性差异(P<0.01)。结论灯草灸对热性惊厥幼年大鼠大脑皮质内Glu与GABA的表达水平具有调节作用。

Objective The objective of this study is to observe the effect of lamp grass moxibustion therapy on the content of glutamic acid(Glu)andγ-aminobutyric acid(GABA)in brain homoplasm of young rats with febrile convulsion and to observe its possible mechanism.Methods Forty healthy SD young rats were randomly divided into model group,control group,diazepan group,lamp grass moxibustion group and diazepan and lamp grass moxibustion group.The rat models of febrile convulsion were made according to literature methods.The content of Glu GABA in brain cortex of rats in each group was detected.Results Compared with control group,Glu content in cerebral cortex of model group was significantly increased(P<0.01).Compared with the model group,the content of Glu in the cerebral cortex of the rats in the diazepan group and the diazepan and lamp grass moxibustion group significantly decreased(P<0.01),and there was no significant difference between the model group and the lamp grass moxibustion group(P>0.05).There was no significant difference between the diazepan group,lamp grass moxibustion group and diazepan and lamp grass moxibustion group(P>0.05),but there was significant difference between the diazepan and lamp grass moxibustion group and the diazepan group(P<0.05),There was extremely significant difference between the diazepan and lamp grass moxibustion group and diazepan group(P<0.01).Compared with the control group,the content of GABA in cerebral cortex of model group significantly decreased(P<0.01).There was a significant difference between model group and diazepan group(P<0.01).There was no significant difference between the diazepan group and the lampgras moxibustion group(P>0.05).There was a difference between the diazepan group and the lamp grass moxibustion group(P<0.05),and there was a significant difference between the lamp grass moxibustion group and the lamp grass moxibustion group(P<0.01).Conclusion The expression levels of Glu and GABA in cerebral cortex of rats with febrile convulsion can be regulated by the lamp grass moxibustion.