不同温度对油樟种子生理及萌发的影响

Effects of Different Temperatures on Physiology and Germination of Cinnamomum longepaniculatum Seed

油樟种子为顽拗性种子,为解决其萌发率低、采收后不易保存等问题,以不同温度处理(-20℃、2℃、15℃、55℃)油樟种子,分析胚与胚乳显微结构,测定种子相对电导率、含水量、可溶性蛋白含量、抗氧化酶活性及MDA含量。结果表明:2℃低温处理能够提高种子内可溶性蛋白含量与抗氧化酶(SOD、POD、CAT)活性,有效降低含水量、MDA含量和相对电导率。因此,2℃处理能够有效保持种子活性,提高种子萌发过程中的各项生理指标,利于油樟种子贮藏与萌发。

The Cinnamomum longepaniculatum seeds are racdcitrous seeds.In order to solve the problem of low germination rate and difficult precervation,we select the seeds as experimental materials and treated with different temperatures(-20℃,2℃,15℃,55℃),then analyze the physiological and anatomical structure changes of the seeds.The results showed:after 2℃treated,the soluble protein content and antioxidant enzyme(SOD,POD,CAT)activity of seeds increased dramatically,but the water content,MDA content and relative conductivity decline sharply.Therefore,2℃could effectively maintain seed activity and improve seed germination.