选择适用于研究滋养层细胞合体化过程的内参基因的实验研究

Selection of internal references gene for studying trophoblast syncytialization

目的筛选并确定适用于滋养层细胞合体化研究的内参基因。方法利用人绒毛膜癌细胞BeWo和人滋养细胞干细胞比较了11个候选内参基因的稳定性,并使用GeNorm、NormFinder和BestKeeper三种软件程序来确定最合适用于研究滋养层细胞合体化的内参基因。结果TBP和RPS13为稳定表达的内参基因,而常用的GAPDH(glyceraldehyde-3-phosphate dehydrogenase)和β-actin(actin,beta)在滋养层细胞合体化过程中表达水平稳定性不佳。结论在利用实时荧光定量聚合酶链反应进行滋养层细胞的合胞化研究时,TBP和RPS13是首选的内参基因。

Objective To screen and identify reference genes suitable for studying on trophoblast cells.Methods The stability of 11 candidate reference genes were compared using BeWo and human trophoblast stem cells(hTSCs).Three software programs,GeNorm,NormFinder,and BestKeeper,were used to determine the most suitable internal reference genes for the study of trophoblast cell syncytialization.Results TBP and GRPS13 were stable reference genes,while GAPDH andβ-actin expression levels were not stable during trophoblast cell syncytialization.Conclusions TBP and RPS13 were recommended as the preferred internal reference genes for trophoblast syncytialization studies using RT-PCR.