辅酶Q10对小鼠脑出血后神经保护作用的实验研究

Neuroprotective effect of coenzyme Q10 on intracerebral hemorrhage in mice

目的:研究辅酶Q10(CoQ10)对脑出血(ICH)小鼠的神经功能、周围组织水肿、炎症因子水平和细胞凋亡的影响。方法:将54只C57WT小鼠随机分为Sham组、ICH组、CoQ10组,采用Ⅳ型胶原酶注射到基底神经节的方法建立脑出血模型,CoQ10组小鼠在脑出血模型建立6 h后,给予CoQ10灌胃处理。于造模后7 d时对所有小鼠进行mNSS评分评估其神经功能,并用公式计算小鼠脑水肿程度;于3 d时采用酶联免疫吸附试验检测炎症因子白介素6(IL-6)及肿瘤坏死因子-α(TNF-α)的表达水平,TUNEL染色法检测细胞凋亡水平。结果:CoQ10治疗降低了脑出血小鼠造模7 d后的mNSS评分(P<0.05),脑组织水含量计算的结果也显示ICH组脑水肿程度加重(P<0.001),而CoQ10组可减轻小鼠的脑水肿(P<0.01)。与ICH组相比,经CoQ10处理可以显著降低炎症因子的表达水平(P<0.01)及减少细胞凋亡水平(P<0.001)。结论:CoQ10可减轻脑出血小鼠神经功能障碍、降低脑水肿程度、减少炎症因子产生、改善细胞凋亡。

Objective:To study the effects of coenzyme Q10(CoQ10)on nerve function,surrounding tissue edema,inflammatory factor levels and apoptosis of intracerebral hemorrhage(ICH)mice.Methods:Fifty-four C57 WT mice were randomly divided into Sham group,ICH group and CoQ10 group.ICH group and CoQ10 group were injected with typeⅣcollagenase into basal ganglia to establish ICH model.CoQ10 group was given CoQ10 intragastric treatment 6 hours after the establishment of ICH model.At 7 days after modeling,all mice were evaluated by mNSS score,and the degree of cerebral edema was calculated by formula.At 3 days,the expression levels of inflammatory factors were detected by enzyme-linked immunosorbent assay,and the apoptosis level was detected by TUNEL staining.Results:CoQ10 treatment reduced the mNSS score(P<0.05),the calculation results of brain water content also showed that the degree of cerebral edema increased in the ICH group(P<0.001),while CoQ10 reduced cerebral edema in mice(P<0.01).Compared with ICH group,CoQ10 could significantly reduce the expression level of inflammatory factors and decrease apoptosis levels(all P<0.001).Conclusion:CoQ10 can relieve the neurological dysfunction,reduce the degree of cerebral edema,reduce the production of inflammatory factors and improve cell apoptosis in mice with ICH.