手性金属有机笼MOC-PA作为毛细管电泳手性固定相

Chiral Metal-organic Cage MOC-PA as a Chiral Stationary Phase for Capillary Electrophoresis

报道了一种手性金属有机笼[Cu(L)(HO)](PA=L-苯丙氨酸,MOC-PA)作为毛细管电泳分离新型固定相的研究.采用X射线粉末衍射仪、红外光谱、热重分析和扫描电子显微镜对该材料进行了表征,结果表明该手性金属有机笼具有良好的热稳定性和空间结构.利用该材料制备的毛细管色谱柱具有良好的手性识别能力,可拆分1,2-二苯基乙二醇、佐匹克隆、4-甲基二苯基甲醇和茴香偶姻等手性药物,也能拆分位置异构体硝基苯酚.通过对拆分1,2-二苯基乙二醇、佐匹克隆、4-甲基二苯基甲醇和茴香偶姻进行最佳条件的探究,得出电压、缓冲溶液浓度及p H值在毛细管电泳中对手性样品分离度的影响.采用佐匹克隆进行毛细管柱的重现性研究表明,用该材料制备的毛细管电泳色谱柱具有一定的稳定性和重现性.可见,金属有机笼是一种良好的毛细管电泳手性固定相,有一定的手性拆分能力,在色谱分离中有较好的发展前景.

A chiral metal-organic cage,[Cu_(12)(L_(PA))_(12)(H_(2)O)_(12)](PA=L-phenylalanine,MOC-PA),was used as a novel stationary phase for capillary electrophoresis separation. The material was characterized by X-ray powder diffractometer,infrared spectroscopy,thermogravimetric analysis and scanning electron microscope. The results showed that the chiral metal-organic cage has good thermal stability and spatial structure. The capillary chromatographic column prepared from this material has a good chiral recognition ability,which can resolve 1,2-diphenylethylene glycol,zopiclone,4-methyldiphenyl methanol and anisin,as well as nitrophenol. By exploring the optimal conditions for 1,2-diphenylethylene glycol,zopiclone,4-methyldiphenylcarbinol and anisoin,the effects of voltage,buffer solution concentration and pH on the resolution of chiral samples in capillary electrophoresis were obtained.And the reprodu-cibility study of the capillary column with zopiclone shows that the capillary electrophoresis column prepared from this material has specific stability and reproducibility. Therefore,the metal organic cage is a good chiral stationary phase in capillary electrophoresis,and has certain chiral separation ability and outstanding development prospect in chromatographic separation.