CMTM6表达与肝细胞癌患者术后复发风险相关性的研究

Correlation between expression of CMTM6 and risk of postoperative recurrence in patients with hepatocellular carcinoma

目的探讨跨膜结构域的趋化素样因子6(CMTM6)表达水平与肝细胞癌(HCC)患者术后复发风险的相关性。方法选择2019年4月至2020年7月中国人民解放军联勤保障部队第九四〇医院收治并行肝切除术的82例HCC患者,收集患者的临床资料,采用PCR法检测肿瘤组织中CMTM6 mRNA的相对表达量。所有患者术后均随访1年,根据是否复发,将患者分为复发组和未复发组,分析两组临床病理特征的差异。以CMTM6 mRNA相对表达量的中位数作为最佳截断值,绘制生存曲线,分析不同CMTM6 mRNA表达水平的HCC患者术后的复发风险。采用多因素logistic回归模型分析HCC患者术后复发的独立危险因素。结果随访截止时间为2021年7月31日,共有34例(41.46%)患者复发。比较复发组与未复发组的临床病理特征,结果显示复发组中甲胎蛋白(AFP)>400 ng/mL患者占比、肝硬化患者占比、肿瘤低分化患者占比、白蛋白(ALB)<35 g/L患者占比,以及肿瘤组织中CMTM6 mRNA的相对表达量均高于未复发组,差异均有统计学意义(P均<0.05)。生存曲线分析显示,CMTM6 mRNA高表达组患者的术后复发风险明显高于CMTM6 mRNA低表达组(log-rankχ^(2)=5.782,P=0.016)。多因素logistic回归模型分析结果显示,AFP>400 ng/mL(OR=65.263)、肝硬化(OR=3.166)、肿瘤低分化(OR=21.005)、ALB<35 g/L(OR=14.995)和CMTM6 mRNA高表达(OR=89.751)均是HCC患者术后复发的独立危险因素(P均<0.05)。结论CMTM6高表达与HCC术后复发有显著相关性,CMTM6可能是潜在的HCC患者的预后预测指标和治疗靶点。

Objective This paper intends to investigate the correlation between the expression of CKLF-like MARVEL transmembrane domain-containing 6(CMTM6)in the transmembrane domain and the risk of postoperative recurrence in patients with hepatocellular carcinoma(HCC).Methods A total of 82 HCC patients who underwent hepatectomy were selected from the No.940 Hospital of the Joint Support Force of the Chinese People's Liberation Army from April 2019 to July 2020.The clinical data of the patients was collected,and the relative expression of CMTM6 mRNA in their tumor tissues was detected by PCR.All patients were followed up for 1 year after operation,and the patients were assigned to the recurrence group and the non-recurrence group according to whether recurrence occurred,and the differences in clinicopathological characteristics of the two groups were analyzed.Using the median relative expression of CMTM6 mRNA as the optimal cutoff value,the survival curve was drawn to analyze the postoperative recurrence risk of HCC patients with different CMTM6 mRNA expressions.The multivariate logistic regression model was used to analyze the independent risk factors of postoperative recurrence in HCC patients.Results The cut-off time of follow-up was July 31,2021,and a total of 34 patients(41.46%)relapsed.A comparison of the clinicopathological characteristics of the recurrence group and the nonrecurrence group shows that in the recurrence group,the proportion of patients with AFP>400 ng/mL,the proportion of patients with liver cirrhosis,the proportion of patients with poorly differentiated tumors,the proportion of albumin(ALB)<35 g/L,and the relative expression levels of CMTM6 mRNA in tumor tissue are higher than those in the non-recurrence group,with statistically significant differences(P<0.05).The survival curve analysis indicates that the risk of postoperative recurrence in the high CMTM6 mRNA expression group is significantly higher than that in the low CMTM6 mRNA expression group(log-rankχ^(2)=5.782 and P=0.016).The results of multivariate logistic regression model analysis suggests that AFP>400 ng/mL(OR=65.263),liver cirrhosis(OR=3.166),poorly differentiated tumor(OR=21.005),ALB<35 g/L(OR=14.995)and high expression of CMTM6 mRNA(OR=89.751)are independent risk factors for postoperative recurrence of HCC patients(P<0.05).Conclusion High expression of CMTM6 is significantly correlated with postoperative recurrence of HCC,and CMTM6 may become a potential prognostic predictor and therapeutic target for HCC patients.