基于脂质组学技术探讨丹贝益肺方对特发性纤维化大鼠的作用机制

Mechanism of Danbei Yifei Decoction on Idiopathic Pulmonary Fibrosis Rats Based on Lipidomic Technology

目的:基于脂质组学技术,探讨丹贝益肺方对特发性纤维化大鼠的代谢机制。方法:采用气管内滴入博莱霉素法制备特发性肺纤维化大鼠模型,使用超高效液相色谱-四极杆静电场轨道阱质谱联用技术检测其血清中的脂质变化,鉴定差异离子,寻找潜在生物标志物。结果:脂质组学测定及通路富集分析结果显示,特发性肺纤维化大鼠的异常代谢主要涉及亚油酸代谢、α-亚麻酸代谢、花生四烯酸代谢、甘油酯代谢和甘油磷脂代谢。ROC曲线显示,11个脂质组分与特发性肺纤维化具有明确相关性,丹贝益肺方干预后出现不同程度的回调趋势。结论:丹贝益肺方可能通过调节生物标志物及相关通路达到治疗特发性肺纤维化的目的,为特发性肺纤维化的脂质代谢提供了可靠依据。

OBJECTIVE: To probe into the metabolic mechanism of Danbei Yifei decoction on idiopathic pulmonary fibrosis rats based on lipidomic technology. METHODS: The rat model of idiopathic pulmonary fibrosis was established by intratracheal injection of bleomycin. Serum lipid changes were detected by ultra-high performance liquid chromatography-performance liquid chromatography-quadrupole electrostatic field orbital well mass spectrometry, and differential ions were identified to search for potential biomarkers. RESULTS: Lipidomics and pathway enrichment analysis showed that the abnormal metabolism of the rats with idiopathic pulmonary fibrosis mainly involved linoleic acid metabolism, α-linolenic acid metabolism, arachidonic acid metabolism, triglyceride metabolism and glycerophospholipid metabolism. ROC curves showed that 11 lipid components were clearly correlated with idiopathic pulmonary fibrosis, with the tendency of different degrees of regression after the intervention of Danbei Yilong decoction. CONCLUSIONS: Danbei Yiku decoction may achieve the treatment of idiopathic pulmonary fibrosis by regulating biomarkers and related pathways, providing a reliable basis for lipid metabolism in idiopathic pulmonary fibrosis.