摘要
植物微生物组是维护植物生长发育、提升抗逆防病的重要调控因素。为发挥植物微生物促进烟草生长、改善烟草根区微生态功能作用,本研究从烟草根表分离筛选可培养细菌组,并对不同菌株的促生能力进行测定。结果表明:①从烟草根表分离并鉴定出可培养菌株310株,隶属于31个属,其中主要为芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas);②对比分析发现假单胞菌属、芽孢杆菌属、寡养单胞菌属(Stenotrophomonas)和成对杆菌属(Dyadobacter)为4种供试土壤烟草根表共有的细菌类群;③对进一步筛选得到的16株菌株进行促生能力的测定,发现6株菌具有固氮能力,5株菌产铁载体,4株菌可溶解无机磷,4株菌产IAA;④盆栽试验验证16株菌株的促生效果,其中37.5%的菌株对烟草生长具有显著促进作用,烟草株高、总鲜物质量和地下部干物质量分别比对照提高35.1%、27.9%和30.7%。总之,从烟草根表分离获得多种具有促生能力的菌株,为未来构建促进烟草健康生长的复合菌剂提供了理论基础。
Plant microbiome is an important factor in plant growth,stress resistance and disease prevention.In order to find the role of plant microbiome in promoting growth and improving root microecology of tobacco,in this study,culturable bacteria were isolated from the root surface of tobacco,and the growth promoting ability of different strains was determined.The results showed that:(1)310 culturable strains were isolated from the root surface of tobacco,belonging to 31 genera,in which most are Bacillus and Pseudomonas.(2)Comparative analysis showed that Pseudomonas,Bacillus,Stenotrophomonas,and Dyadobacter are the common bacteria in tobacco root surface of different tested soils.(3)Within 16 strains obtained by further screening,6 strains have the ability to fix nitrogen,5 strains can produce siderophores,4 strains can dissolve inorganic phosphorus,and 4 strains can produce IAA.(4)Pot experiment verified the growth promoting effect of the strains,the six strains increased the plant height,total fresh weight and underground dry weight by at least 35.1%,27.9%and 30.7%,respectively,accounting for 37.5%of all inoculated strains.In short,a variety of strains with growth-promoting ability can be isolated from the root surface of tobacco,which provides a theoretical basis for the construction of microbial community to promote tobacco growth in the future.
作者
刘冬晖
李凤巧
靳志丽
李孝刚
LIU Donghui;LI Fengqiao;JIN Zhili;LI Xiaogang(College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China;Yongzhou Tobacco Company of Hunan Province,Yongzhou,Hunan 425000,China)
出处
《土壤》
CAS
CSCD
北大核心
2022年第4期750-755,共6页
Soils
基金
中国烟草总公司湖南省公司科技项目(18-20Aa03)资助。
关键词
烟草根表
可培养细菌
促生筛选
植物微生物组
Tobacco root surface
Cultivable bacteria
Growth promotion screening
Plant microbiome