整合SV40 Large T抗原基因的人胚肺成纤维细胞IMR90的蛋白质组和代谢组研究

Proteomics and Metabolomics of Human Embryonic Lung Fibroblast IMR90 Integrated with SV40 Large T Antigen Gene

随着高通量技术的发展,基因组学、转录组学、蛋白质组学和代谢组学等组学研究为深入了解细胞新陈代谢的机理提供了新视角。本研究先验证了整合SV40 Large T抗原基因的IMR90细胞——IMRT,表现出衰老表型。通过蛋白质组学与代谢组学相结合的方法,鉴定了IMRT和IMR90细胞中的差异蛋白质和差异代谢物。结果表明:在IMRT细胞中,重要代谢通路的蛋白质水平发生变化,如葡萄糖磷酸变位酶1(PGM1)、6-磷酸葡萄糖脱氢酶(G6PD)、谷胱甘肽过氧化物酶1(GPX1)、谷胱甘肽S-转移酶M2(GSTM2)等。相关通路中的代谢物,如6-磷酸葡萄糖,肉碱,磷酸胆碱,亮氨酸异亮氨酸,谷氨酰胺和丙氨酸的含量有显著变化。结合分析结果,我们在IMR90中沉默GPX1基因,促进了IMR90的衰老进程,表明GPX1在细胞衰老进程中的重要作用。

With the development of high-throughput technology,genomics,transcriptomics,proteomics and metabolomics and other omics research provide a new perspective for in-depth understanding of the mechanism of cell metabolism.This study shows that the IMR90 cells integrated with the SV40 Large T antigen gene,IMRT,exhibit an senescent phenotype.Through a combination of proteomics and metabolomics,the differential proteins and differential metabolites in IMRT and IMR90 cells are identified.The results show that in IMRT cells,the protein levels of important metabolic pathways have significant changes,such as glucose phosphate mutase 1(PGM1),glucose 6-phosphate dehydrogenase(G6PD),glutathione peroxidase 1(GPX1),glutathione S-transferase M2(GSTM2),etc.Metabolites in related pathways,such as glucose 6-phosphate,carnitine,phosphocholine,leucine-isoleucine,glutamine and alanine,have significant changes.Combined with the analysis results,we silence GPX1 in IMR90,which promote the senescence process of IMR90,indicating that GPX1 plays an important role in the cellular senescence process.