包被丁酸钠对蛋鸡生产性能和肠道健康的影响

Effects of Coated Sodium Butyrate on Performance and Intestinal Health of Laying Hens

本试验旨在研究包被丁酸钠(CSB)对蛋鸡生产性能以及肠道pH、消化酶活性和相关基因表达的影响。试验选用800只51周龄健康的罗曼粉蛋鸡,随机分为4个组,每组10个重复,每个重复20只鸡。对照组饲喂基础饲粮,3个试验组分别饲喂在基础饲粮中添加300、500和800 mg/kg CSB的饲粮。试验期12周。结果表明:1)饲粮中添加不同水平CSB可线性提高蛋鸡第5~8周、第9~12周和第1~12周的产蛋率以及第5~8周的平均日采食量(P<0.05),对蛋鸡平均蛋重无显著影响(P>0.05);与对照组相比,饲粮中添加800 mg/kg CSB可显著降低蛋鸡第9~12周和第1~12周的料蛋比(P<0.05);与300 mg/kg CSB添加组相比,饲粮中添加500和800 mg/kg CSB可显著提高蛋鸡第1~4周和第5~8周的合格蛋率(P<0.05)。2)与对照组相比,饲粮中添加800 mg/kg CSB可显著降低蛋鸡回肠pH(P<0.05);饲粮中添加不同水平CSB可线性降低蛋鸡十二指肠和空肠pH(P<0.05)。3)饲粮中添加不同水平CSB可线性提高蛋鸡十二指肠胰蛋白酶和淀粉酶活性(P<0.05)。4)与对照组相比,饲粮中添加500和800 mg/kg CSB可显著降低蛋鸡血清D-乳酸含量(P<0.05)。5)与对照组相比,饲粮中添加不同水平CSB均可显著降低蛋鸡空肠黏膜白细胞介素-1β(IL-1β)mRNA相对表达量(P<0.05),显著提高空肠黏膜白细胞介素-10(IL-10)mRNA相对表达量(P<0.05);饲粮中添加300和500 mg/kg CSB可显著提高蛋鸡空肠黏膜黏蛋白2(MUC2)mRNA相对表达量(P<0.05);饲粮中添加不同水平CSB可线性降低蛋鸡回肠黏膜IL-1βmRNA相对表达量(P<0.05),线性提高蛋鸡空肠和回肠黏膜闭锁小带蛋白-1(ZO-1)以及回肠黏膜MUC2 mRNA相对表达量(P<0.05)。由此可见,饲粮中添加500和800 mg/kg CSB对蛋鸡的生产性能和肠道健康有一定改善作用。

This experiment was conducted to investigate the effects of coated sodium butyrate(CSB)on performance,intestinal pH,digestive enzyme activities and related gene expression of laying hens.A total of 800 healthy Lohmann pink laying hens of 51-week-old were randomly divided into 4 groups with 10 replicates per group and 20 hens per replicate.Hens in the control group were fed a basal diet,and those in 3 experimental groups were fed the basal diet supplemented with 300,500 and 800 mg/kg CSB,respectively.The trial lasted for 12 weeks.The results showed as follows:1)different dietary levels of CSB linearly increased the laying rate of laying hens in weeks 5 to 8,9 to 12 and 1 to 12 as well as the average daily feed intake in weeks 5 to 8(P<0.05),but had no significant effects on the average egg weight of laying hens(P>0.05).Compared with the control group,dietary 800 mg/kg CSB significantly decreased the feed to egg ratio of laying hens in weeks 9 to 12 and 1 to 12(P<0.05).Compared with 300 mg/kg CSB supplemental group,dietary 500 and 800 mg/kg CSB significantly increased the qualified egg rate of laying hens in weeks 1 to 4 and 5 to 8(P<0.05).2)Compared with the control group,dietary 800 mg/kg CSB significantly decreased ileum pH of laying hens(P<0.05);different dietary levels of CSB linearly decreased the pH in duodenum and jejunum of laying hens(P<0.05).3)Different dietary levels of CSB linearly increased the activities of trypsin and amylase in duodenum of laying hens(P<0.05).4)Compared with the control group,dietary 500 and 800 mg/kg CSB significantly decreased serum D-lactic acid content of laying hens(P<0.05).5)Compared with the control group,dietary CSB significantly decreased the mRNA relative expression level of interleukin-1β(IL-1β)in jejunum mucosa of laying hens(P<0.05),and significantly increased the mRNA relative expression level of interleukin-10(IL-10)in jejunum mucosa of laying hens(P<0.05);dietary 300 and 500 mg/kg CSB significantly increased the mRNA relative expression level of mucin 2(MUC2)in jejunum mucosa of laying hens(P<0.05);different dietary levels of CSB linearly decreased the mRNA relative expression level of IL-1βin ileum mucosa of laying hens(P<0.05),and linearly increased the mRNA relative expression level of zonula occluden-1(ZO-1)in jejunum and ileum mucosa and the mRNA relative expression level of MUC2 in ileum mucosa of laying hens(P<0.05).In conclusion,dietary 500 and 800 mg/kg CSB can improve the performance and intestinal health of laying hens.