高效液相色谱法测定辐状肋柱花中10种成分的含量

Determination of 10 components in Lomatogonium rotatum by HPLC

目的 建立高效液相色谱法同时测定辐状肋柱花中马钱苷酸、獐芽菜苦苷、当药苷、芦丁、当药黄素、木犀草素、苦龙胆脂苷、当药醇苷、槲皮素、8-羟基-1,3,5-三甲氧基[口山]酮的含量。方法 采用Waters SunFirODS C_(18)柱(4.6 mm×250 mm,5 μm),乙腈-0.05%磷酸溶液为流动相,进行梯度洗脱,流速1.0 mL·min^(-1),检测波长 254 nm,柱温 30℃。结果 10种成分马钱苷酸、獐芽菜苦苷、当药苷、芦丁、当药黄素、木犀草素、苦龙胆脂苷、当药醇苷、槲皮素、8-羟基-1,3,5-三甲氧基[口山]酮均得到良好分离,线性关系良好(r≥0.9993),加样回收率均在 96.20%~100.68%,RSD<2.9%。结论 该方法快捷简便、稳定可靠,可应用于辐状肋柱花及其制剂的质量控制,为建立藏药辐状肋柱花质量标准提供依据。

Objective To simultaneously determine loganin,swertiamarin,sweroside,rutin,swertisin,luteolin,genticoside,mercaptoside,quercetin,and 8-hydroxy-1,3,5-trimethoxyxanthone in Lomatogonium rotatum by HPLC.Methods Waters SunFirODS C_(18) column (4.6 mm×250 mm,5 μm) was used.Acetonitril-0.05% phosphoric acid solution was the mobile phase with gradient elution.The volume flow rate was 1.0 mL·min^(-1),the detection wavelength was 254 nm and the column temperature was 30℃.Results The 10 components (loganin,swertiamarin,sweroside,rutin,swertisin,luteolin,genticoside,mercaptoside,quercetin,and 8-hydroxy-1,3,5-trimethoxyxanthone) were well separated and showed good linear relationship (r ≥0.9993).The recoveries ranged 96.20%-100.68%,RSD <2.9%.Conclusion The method is fast,simple,stable and reliable.It can be used for the quality control of Lomatogonium rotatum and its preparations,providing a basis for establishing the quality standard of Tibetan medicine Lomatogonium rotatum.