探讨不同浓度Matrigel对小鼠Lewis肺癌异位皮下移植瘤的影响

To investigate the effect of different concentrations of Matrigel on ectopic subcutaneous transplanted tumor of Lewis lung cancer in mice

目的探讨不同浓度Matrigel对小鼠Lewis肺癌异位皮下移植瘤的影响。方法将24只C57BL/6J Nifdc小鼠随机分为肺癌组、50%基质胶组、75%基质胶组、100%基质胶组,每组各6只。将Lewis肺癌细胞(LL/2)分别与0%、50%、75%、100%Matrigel按照体积1∶1混合,于小鼠右侧前肢腋下进行皮下注射,每日测量各组小鼠体重、饮食量及饮水量;观察比较各组成瘤时间、成瘤率和肿瘤大小,于肿瘤形成后每天测量肿瘤的长径、短径,计算肿瘤体积变化;于肿瘤接种第15天处死动物,完整剥离肿瘤组织并称重,HE染色检测肿瘤组织病变情况。结果与肺癌组相比,75%基质胶组小鼠自造模第2天起体重显著升高(P<0.05或P<0.01);100%基质胶组小鼠自造模第10天起,体重明显上升(P<0.05或P<0.01)。各组小鼠平均饮食量于造模8 d后有所降低,饮水量总体呈现上升趋势。各组小鼠成瘤率均为100%,添加基质胶组的小鼠相比肺癌组小鼠成瘤时间早,肿瘤生长速度快,且肿瘤较大。其中,75%基质胶组肿瘤体积增长最快,肿瘤重量最大,为(1358.88±388.14)mg,与肿瘤组相比,具有显著性差异(P<0.05);100%基质胶组肿瘤体积增长次之,肿瘤重量为(1142.37±423.08)mg,与肿瘤组相比,具有显著性差异(P<0.05);50%基质胶组肿瘤体积增长最慢,肿瘤重量最小,为(808.83±393.41)mg。HE染色结果显示,相比于肺癌组,添加基质胶组的肿瘤细胞生长旺盛、轮廓清晰、细胞核体积较大,肿瘤组织间质中血管成分明显增多。结论基质胶能稳定成瘤率,加快肿瘤生长,增大成瘤体积,且75%基质胶浓度条件下肿瘤增长最快、肿瘤体积最大,肿瘤的均一性较好。

Objective To investigate the effect of different concentrations of Matrigel on ectopic subcutaneous transplanted tumor of Lewis lung cancer in mice.Methods Twenty-four C57BL/6J Nifdc mice were randomly divided into a Lung cancer group,50%Matrigel group,75%Matrigel group,and 100%Matrigel group,with six mice in each group.Lewis lung cancer cells(LL/2)were mixed with 0%,50%,75%and 100%Matrigel at a volume ratio of 1∶1.The mice were injected subcutaneously under the armpit of the right forelimb.The weight,diet and volume of drinking water of mice in each group were measured every day.The tumor formation time,tumor formation rate and tumor size were observed.The long diameter and short diameter of the tumor were measured every day after the formation of the tumor,and the change in tumor volume was calculated.The animals were terminated on 15 days after tumor inoculation,the tumor tissue was removed and weighed,and the pathological changes of the tumor tissue were detected by HE staining.Results Compared with the Lung cancer group,the weight of the 75%Matrigel group increased significantly from the second day of modeling(P<0.05 or P<0.01).The weight of mice in the 100%Matrigel group increased significantly from the 10th day of modeling(P<0.05 or P<0.01).The average diet of mice in each group decreased eight days after modeling,and the volume of drinking water showed an upward trend.The tumor formation rate in each group was 100%.Mice with Matrigel had earlier tumor formation time,faster tumor growth rate,and a larger tumor than those without.Among them,the 75%Matrigel group had the fastest tumor volume growth and the greatest tumor weight,(1358.88±388.14)mg,compared with the Lung cancer group(P<0.05).The tumor volume growth and tumor weight in the 100%Matrigel group were the second greatest,and the tumor weight was(1142.37±423.08)mg,which was significantly different from that in the Lung cancer group(P<0.05).The tumor volume growth and tumor weight in the 50%Matrigel group were the smallest,(808.83±393.41)mg.The result of HE staining show that,compared with the Lung cancer group,the tumor cells with Matrigel group had vigorous growth,a clear outline,large nuclear volume,and significantly more vascular components in the stroma of the tumor tissue.Conclusions Matrigel can stabilize the tumor formation rate,accelerate tumor growth,and increase the tumor formation volume.Under the condition with 75%Matrigel,the tumor growth was the fastest,the tumor volume was the largest,and the tumor was uniform.