摘要
目的:探讨芪苈强心胶囊对心肌梗死(MI)大鼠心肌组织纤维化的影响及可能的作用机制。方法:采用永久性左冠状动脉前降支结扎方法建立心肌梗死大鼠模型,术后随机分组为模型组、芪苈强心胶囊组、卡托普利组,另外设假手术组只穿线但不接扎作为平行对照。药物治疗4周后,采用苏木素伊红(HE)染色观察心脏病理形态,Masson染色观察心肌组织纤维化并计算胶原容积分数(CVF)。实时荧光PCR观察miR-133a、转化生长因子β1(TGF-β1)、Smad2、Smad3、Ⅰ型胶原(col-Ⅰ)、Ⅲ型胶原(col-Ⅲ)mRNA表达量。结果:与假手术组比较,模型组心肌细胞排列紊乱,CVF显著升高(P<0.01),col-Ⅰ、col-ⅢmRNA表达水平明显增加(P<0.01);miR-133a表达水平显著降低(P<0.01),TGF-β1、Smad2、Smad3 mRNA表达水平明显升高(P<0.05,P<0.01)。与模型组比较,芪苈强心胶囊组和卡托普利组梗死边缘区心肌细胞排列相对规整,CVF显著下降(P<0.05);miR-133a表达水平明显提高(P<0.05~0.01),TGF-β1、Smad2、Smad3 mRNA表达水平显著降低(P<0.01)。结论:芪苈强心胶囊可改善心肌梗死大鼠心肌组织纤维化,其作用机制与调节miRNA-133a/TGF-β1/Smads信号通路基因表达有关。
Objective:To investigate the effect and possible mechanism of Qiliqiangxin capsule on myocardial fibrosis in rats with myocardial infarction(MI).Methods:The rat model of myocardial infarction was established by ligation of anterior descending branch of left coronary artery.The rats were randomly divided into model group,Qiliqiangxin capsule group,captopril group after operation.Rats that without ligation were set as parallel control group,namely,the sham group.Cardiac pathology was observed by hematoxylin eosin(HE)staining after 4 weeks of treatment.Masson staining was used to observe myocardial fibrosis and calculate collagen volume fraction(CVF).The miR-133a and expression levels of transforming growth factorβ1(TGF-β1),Smad 2,Smad 3,typeⅠcollagen(col-Ⅰ),typeⅢcollagen(col-Ⅲ)mRNA were examined by Real-time PCR.Results:Compared with the sham group,myocardial cells in model group were disordered,CVF was significantly increased(P<0.01),the expression levels of col-Ⅰ,col-ⅢmRNA were increased(P<0.01);besides,the expression level of miR-133a was significantly decreased(P<0.01),the expression levels of TGF-β1,Smad 2 and Smad 3 mRNA were increased(P<0.05,P<0.01).Compared with the model group,the arrangement of myocardial cells in Qiliqiangxin capsule group and captopril group were orderly and CVF was significantly decreased(P<0.05);the expression level of miR-133a was increased(P<0.05,P<0.01),the expression levels of TGF-β1,Smad 2 and Smad 3 mRNA were significantly decreased(P<0.01).Conclusion:Qiliqiangxin capsule can improve myocardial infarction rat myocardial tissue fibrosis,its mechanism may be in related with the regulation on miR-133a/TGF-β1/Smads signal pathway at the genetic level.
作者
纪晓迪
吴爱明
吕梦
杨丁
娄利霞
聂波
赵久丽
赵明镜
JI Xiao-di;WU Ai-ming;LV Meng;YANG Ding;LOU Li-xia;NIE Bo;ZHAO Jiu-li;ZHAO Ming-jing(Dongzhimen Hospital,Beijing University of Chinese Medicine,Ministry of Education and Beijing Key Laboratory of Traditional Chinese Medicine Internal Medicine,Beijing 100700,China)
出处
《海南医学院学报》
CAS
2022年第21期1608-1613,共6页
Journal of Hainan Medical University
基金
国家自然科学基金资助项目(81973787)。