多色免疫荧光技术在胃癌免疫治疗中的临床应用

Clinical Application of Multiplex Immunofluorescence Technique in Immunotherapy of Gastric Cancer

胃癌是我国常见的恶性肿瘤,其治疗现已进入免疫治疗时代。多色免疫荧光技术是近年出现的一项新兴检测技术,包括多色免疫荧光染色、多光谱成像和组织软件定量分析三个环节,能对肿瘤免疫微环境中特异性免疫细胞群进行共定位分析。国内外已有大量研究利用多色免疫荧光技术实现胃癌免疫微环境中不同细胞亚群及其分子表达的检测,并在胃癌治疗和预后评估方面显示出重要的临床价值。尽管存在一定局限性,但与传统方法相比,该技术具有高重复性、高效率、高成本效益等优点。本文就多色免疫荧光技术在胃癌免疫治疗中的临床应用作一综述。

Gastric cancer is a common malignant tumor in China.The treatment of gastric cancer has entered the era of immunotherapy.Multiplex immunofluorescence technology is a new detection technology in recent years,including multiplex immunofluorescence staining,multispectral imaging and tissue quantitative analysis,which can co-locate specific immune cell populations in tumor immune microenvironment.A large number of studies used multiplex immunofluorescence technology to detect different cell subsets and their molecular expression in the immune microenvironment of gastric cancer,which had shown important clinical value in the treatment and prognosis evaluation of gastric cancer.This technique has certain limitation,but it has much advantages,such as high repeatability,high efficiency and high cost-effectiveness compared with traditional methods.This article reviewed the clinical application of multiplex immunofluorescence technique in immunotherapy of gastric cancer.