长链非编码RNA-Keratin 8的调控通路分析

Regulatory Pathway Analysis of Long Non-coding RNA-Keratin 8

目的运用生物信息学分析方法,分析长链非编码RNA-细胞角蛋白8(KRT8)在细胞内的调控靶基因及信号通路,并对其进行生物学功能注释,阐述lncRNA-KRT8在细胞内的功能作用。方法在miRDB数据库和Starbase3.0数据库中预测与lncRNA-KRT8有miRNA-lncRNA互作关系的miRNA,将结果取交集;在Starbase3.0数据库中预测miRNAs靶基因,并进行GO功能注释和Pathway通路富集。结果在miRDB数据库预测到15个miRNAs与lncRNA-KRT8相作用,Starbase3.0数据库预测到60个miRNAs与之相作用,取2个数据库的交集共获得2个miRNAs;GO功能注释显示lncRNA-KRT8通过hsa-miR-150-5p作用的靶基因主要调控蛋白结合、RNA结合、金属离子结合信号通路、转录调控作用及蛋白质的磷酸化过程;通过hsa-miR-512-3p作用的靶基因主要调控蛋白结合、DNA结合、金属离子的结合信号通路、蛋白质的泛素化降解过程;通过hsa-miR-150-5p主要调控ErbB信号通路、肿瘤中糖蛋白信号通路、miRNAs、胃癌信号通路等;通过hsa-miR-512-3p作用的信号通路主要是乙型肝炎、人T细胞白血病病毒、前列腺癌、胰岛素抵抗和MAPK信号通路等。结论长链非编码RNA-KRT8在细胞内的调控作用具体与蛋白质的结合和金属离子结合及糖蛋白、脂代谢信号通路密切相关,其可通过上述作用通路调控疾病的病理进展。

Objective To analyze the regulatory target genes and signaling pathways of long non-coding RNA-cytokeratin 8(KRT8)in cells by bioinformatics analysis,and to annotate its biological functions,so as to elucidate the functional role of lncRNA-KRT8 in cells.Methods The miRNAs interacting with lncRNA-KRT8 were predicted in miRDB database and Starbase3.0 database,and the results were intersected.Target genes of miRNAs were predicted in the Starbase 3.0 database,and GO functional annotation and Pathway enrichment were performed.Results A total of 15 miRNAs were predicted to interact with lncRNA-KRT8 in miRDB database,and 60 miRNAs were predicted to interact with lncRNA-KRT8 in Starbase database.A total of 2 miRNAs from the intersection of the two databases were selected for further analysis.GO functional annotation showed that the target genes of long non-coding RNA KRT8 through hsa-miR-150-5p mainly regulated protein binding,RNA binding,metal ion binding signaling pathway,transcriptional regulation and protein phosphorylation.The target genes of hsa-miR-512-3p mainly regulate protein binding,DNA binding,metal ion binding signaling pathway,protein ubiquitination degradation process;hsa-miR-150-5p mainly regulates ErbB signaling pathway,tumor glycoprotein signaling pathway,miRNAs,gastric cancer signaling pathway,etc.The signaling pathways through hsa-miR-512-3p are mainly hepatitis B,human T cell leukemia virus,prostate cancer,insulin resistance and MAPK signaling pathways.Conclusion The regulation of long non-coding RNA-KRT8 in cells is closely related to protein binding and metal ion binding and glycoprotein and lipid metabolism signaling pathways,which can regulate the pathological progression of diseases through the above-mentioned pathways.