mTOR、PTEN、Ki-67蛋白表达水平变化与结肠癌病理学特征的相关性分析

Correlation of mTOR, PTEN and Ki-67 Protein Expression with Pathological Features of Colon Cancer

目的探讨哺乳动物雷帕霉素靶蛋白(mTOR)、张力蛋白同源蛋白(PTEN)、Ki-67蛋白表达与结肠癌病理学特征的相关性。方法收集2013年3月-2016年6月我院病理科收集的90例结肠癌组织标本(结肠癌组)、45例正常结肠组织(对照组),采用免疫组化染色检测两组标本中mTOR、PTEN、Ki-67蛋白的表达情况,并分析三种蛋白不同的淋巴结转移、TNM分期、肿瘤分化程度结肠癌组织中的表达差异。结果结肠癌组mTOR蛋白、Ki-67蛋白阳性表达率分别为71.11%、74.44%,高于对照组的13.33%、20.00%,差异有统计学意义(P<0.05);结肠癌组PTEN蛋白阳性表达率为23.33%,低于对照组的93.33%,差异有统计学意义(P<0.05);不同TNM分期、是否发生淋巴结转移、不同肿瘤分化的结肠癌组织中mTOR蛋白、PTEN蛋白、Ki-67蛋白阳性表达率分别比较,差异有统计学意义(P<0.05)。结论结肠癌组织中mTOR蛋白、Ki-67蛋白高表达,PTEN蛋白低表达,并且与肿瘤的发生发展关系密切。

Objective To investigate the relationship between the expression of mammalian target of rapamycin(mTOR),phosphatase and tensin homologue deleted on chromosome(PTEN),Ki-67 protein and the pathological features of colon cancer.Methods From March 2013 to June 2016,90 cases of colon cancer tissues(colon cancer group)and 45 cases of normal colon tissues(control group)were collected from the Department of Pathology of our hospital.The expression of mTOR,PTEN and Ki-67 protein in the two groups was detected by immunohistochemical staining,and the expression differences of the three proteins in different lymph node metastasis,TNM stage and tumor differentiation were analyzed.Results The positive expression rates of mTOR protein and Ki-67 protein in colon cancer group were 71.11%and 74.44%respectively,which were higher than 13.33%and 20.00%in control group,and the differences were statistically significant(P<0.05).The positive expression rate of PTEN protein in colon cancer group was 23.33%,which was lower than 93.33%in control group,and the difference was statistically significant(P<0.05).The positive expression rates of mTOR protein,PTEN protein and Ki-67 protein in colon cancer tissues with different TNM stages,lymph node metastasis and tumor differentiation were significantly different(P<0.05).Conclusion mTOR protein and Ki-67 protein are highly expressed in colon cancer tissues,and PTEN protein is lowly expressed,which is closely related to the occurrence and development of tumors..