HPV感染及IL-1β、EGFR单核苷酸多态性与非吸烟女性肺癌的相关性研究

Correlation between HPV infection and single nucleotide polymorphisms of IL-1βand EGFR and lung cancer in non-smokers female

目的探讨HPV感染及IL-1β、EGFR单核苷酸多态性与非吸烟女性肺癌发病的相关性。方法采用病例对照研究方法,纳入非吸烟女性肺癌患者197例和健康女性87例。采用PCR技术检测HPV-DNA,采用TaqMan单核酸多态性分型技术检测IL-1β-31多态性(-31C/T、-511T/C)及EGFR多态性(-216G/T、-191C/A、8227G/A、D994D和R497K)。采用非条件logistic回归分析多态基因型与非吸烟女性肺癌间相关性。结果非吸烟女性肺癌患者HPV 16检出率显著高于对照组(P<0.05);两组IL-1β-511 T/C及EGFR-191 C/A、-216 G/T、D99D、R497K位点单核苷酸多态性差异无统计学意义(P>0.05),两组IL-1β-31 C/T位点单核苷酸多态性差异有统计学意义(P<0.05),T/T基因型肺癌发生风险显著增加(OR=0.25,95%CI:0.16~0.55)。两组EGFR基因的-191C/A、-216G/T、D99D和R497K基因多态性差异无统计学意义(P>0.05),两组EGFR-8227GA单核苷酸多态性差异有统计学意义(P<0.05),G/A基因型肺癌发生风险显著增加(OR=0.22,95%CI:0.18~0.57)。结论HPV 16感染及IL-1β-31位点T/T基因型、EGFR基因-8227位点G/A基因型是非吸烟女性肺癌发生的高风险因素。

Objective To explore the relationship between HPV infection and polymorphisms of IL-1βand EGFR and lung cancer in non-smokers female.Methods A case-control study method was used,with 197 patients with lung cancer of non-smoking female lung cancer patients and 87 healthy female.The HPV DNA was detected with PCR technology,and the polymorphisms of IL-1β(-31C/T、-511T/C)and EGFR(-216G/T、-191C/A、8227G/A、D994D and R497K)were detected with TaqMan single nucleic acid polymorphism typing technology.Non-conditional Logistic regression was used to analyze the correlation between polymorphic genotype and lung cancer in non-smokers.Results The detection rate of HPV 16 in non-smoking female with lung cancer was significantly higher than that in the control group(P<0.05).There was no significant difference in the gene polymorphisms of IL-1β-511 T/C and EGFR-191 C/A,-216 G/T,D99D,and R497K between the two groups(P>0.05).There was statistically significant difference in IL-1β-31 C/T locus gene polymorphism between the two groups(P<0.05),andthe risk of T/T genotype lung cancer was significantly increased(OR=0.25,95%CI:0.16~0.55).There was no statistically significant difference in the-191C/A,-216G/T,D99D and R497K gene polymorphisms of the EGFR gene between the two groups(P>0.05).there was statistically significant difference in EGFR-8227GA gene polymorphism between the two groups(P<0.05),and the risk of G/A genotype lung cancer was significantly increased(OR=0.22,95%CI:0.18-0.57).Conclusions HPV 16 infection,IL-1β-31 T/T genotype,and EGFR-8227 G/A genotype are high risk factors for lung cancer in non-smoking female.