基于Cu_(2)O/AuNPs构建电流型免疫传感器检测载脂蛋白A1

An amperometric immunosensor based on Cu_(2)O/AuNPs for detection of apolipoprotein-A1

通过溶剂热法制备氧化亚铜(Cu_(2)O)纳米立方体,由原位还原氯金酸得到氧化亚铜/金纳米复合物(Cu_(2)O/AuNPs),并将其修饰于玻碳电极后,利用壳聚糖和戊二醛的酰胺键连接作用将膀胱癌肿瘤标志物载脂蛋白A1的重组抗体(Ab)固定于电极表面制成电流型免疫传感器,当载脂蛋白A1的抗原(apolipoprotein-A1,Apo-A1)与抗体发生免疫反应后,依据Cu_(2)O氧化峰电流的降低实现对Apo-A1的定量检测.实验结果表明:在最佳实验条件下,Cu_(2)O氧化峰电流变化值与Apo-A1浓度对数在10.0 pg·mL^(-1)~10.0 g·mL^(-1)浓度范围内呈良好的线性关系.该方法成功用于实际样品检测,对膀胱癌的临床诊断具有重要意义.

Cuprous oxide(Cu_(2)O)nanocube is prepared by solvothermal method,and chloroauric acid is further in-situ reduced to obtain cuprous oxide/gold nanocomposites(Cu_(2)O/AuNPs),which is modified on the glassy carbon electrode.The recombinant antibody(Ab)of apolipoprotein-A1(denoted as Apo-A1)is fixed on the surface of the electrode using the amide bond between chitosan and glutaraldehyde to fabricate an amperometric immunosensor.When the immunoreaction between Apo-A1 and Ab occures,the quantitative detection of Apo-A1 is realized based on the change of the oxidation peak current for Cu_(2)O decreased.The experimental results show that good linear relationship between the change of the oxidation peak for Cu_(2)O and the Apo-A1 concentration is obtained in the range of 10.0 pg·mL^(-1)~10.0μg·mL^(-1) under optimal conditions.The proposed method is successfully used to detect Apo-A1 in real samples with great significance to the clinical diagnosis of bladder cancer.