摘要
从重金属矿山土壤中富集混合菌用于含镉(Cd(Ⅱ))废水处理。批量吸附试验结果表明,适宜的pH、温度、初始Cd(Ⅱ)浓度和投加量分别为6.0、30℃、20 mg/L和1 g/L。在最适条件下,活性混合菌对Cd(Ⅱ)的去除效率(91.97%)高于灭活混合菌(79.54%)。活性混合菌的吸附等温线和动力学过程分别符合Langmuir模型和准一级动力学模型。FTIR结果表明,胺基、羟基和磷酸在Cd(Ⅱ)的吸附中发挥重要作用;XRD结果表明,反应后存在Cd(OH)_(2)和CdO晶体。处理含Cd(Ⅱ)废水后,混合菌中的优势菌属包括Comamonas(39.94%)、unclassified_f__Enterobacteriaceae(34.96%)、Ochrobactrum(14.07%)、Alcaligenes(4.84%)、Bordetella(2.07%)、Serratia(1.04%)和Bacillus(1.01%)。功能预测表明,与代谢相关的基因丰度发生显著变化。该研究表明,混合菌群具有处理含镉废水的潜力。
Mixed bacteria were enriched from heavy metals mine soil for cadmium(Cd(Ⅱ))-containing wastewater treatment. Batch adsorption experiment results showed that the optimal pH, temperature, initial Cd(Ⅱ) concentration, and biomass dosage were 6.0, 30 ℃, 20 mg/L, and 1 g/L, respectively. Living biomass exhibited better Cd(Ⅱ) removal efficiency(91.97%) than autoclaved biomass(79.54%) under optimal conditions. The isotherms and kinetics of living biomass conformed to the Langmuir isotherm model and pseudo-first-order kinetic model, respectively. FTIR results implied that amine groups, hydroxyl groups and phosphoric acid play an important role in the Cd(Ⅱ) adsorption process, while XRD results showed that crystalline Cd(OH)and CdO were obtained. After Cd(Ⅱ)-containing wastewater treatment exposure, the dominant bacteria genera included Comamonas(39.94%), unclassified_f__Enterobacteriaceae(34.96%), Ochrobactrum(14.07%), Alcaligenes(4.84%), Bordetella(2.07%), Serratia(1.04%), and Bacillus(1.01%). Function prediction showed that the abundance of metabolic genes changed significantly. This study proposes the potential application of mixed bacteria for Cd(Ⅱ)-containing wastewater treatment.
作者
曾涛涛
张晓玲
农海杜
胡青
王亮钦
王爱杰
Tao-tao ZENG;Xiao-ling ZHANG;Hai-du NONG;Qing HU;Liang-qin WANG;Ai-jie WANG(Hunan Provincial Key Laboratory of Pollution Control and Resources Reuse Technology,University of South China,Hengyang 421001,China;Key Laboratory of Environmental Biotechnology,Chinese Academy of Sciences,Beijing 100085,China)
基金
supported by the National Natural Science Foundation of China (No. 52170164)
the Open Project of Key Laboratory of Environmental Biotechnology,CAS (No. kf2018001)
the Scientific Research Foundation for Returned Scholars at the University of South China (No. 2018XQD25)
关键词
Cd(Ⅱ)
废水处理
细菌群落
功能预测
Cd(Ⅱ)
wastewater treatment
bacterial community
function prediction
