制药废水活性污泥中高效异养硝化-好氧反硝化菌分离及特性研究

Isolation and identification of highly efficient heterotrophic nitrification-aerobic denitrification strains from the pharmaceutical waste water activate sludge and the study of their capabilities

目的从制药废水活性污泥中筛选高效异养硝化好氧反硝化菌株。方法通过富集培养,BTB平板培养基快速筛选,纯化株通过形态生理生化鉴定和16S rRNA分析确定分类地位。在好氧条件下,对筛选株进行脱氮性能研究以及pH、碳源及含量和基础氨氮耐受进行考察。结果获取PWAS17,PWAS21和PWAS343株异氧硝化好氧反硝化菌株。初步鉴定PWAS17为水生产碱杆菌(Alcaligenes aquatilis)、PWAS21为蜡状芽胞杆菌(Bacillus cereus),PWAS34为斯惠假单胞菌(Pseudomonas sihuiensis)。其中Alcaligenes aquatilis和Pseudomonas sihuiensis少有报道。针对3株菌脱氮性能进行研究,结果表明三株菌均能在去除氨氮同时降解COD,并且在去除氨氮过程中几乎无硝基氮和亚硝基氮的积累,均具有异养硝化好氧反硝化特性。通过对菌株脱氮条件优化,得出当pH为9.0,以0.8%柠檬酸钠为碳源,摇床转速220 r/min,接种量5%,温度30℃时最高氨氮去除率,PWAS17最高达92.1%,PWAS21最高达88.7%,PWAS34最高达92.1%。且基础氨氮浓度在50~500 mg/L范围内对3株菌的去除氨氮能力没有明显的抑制。

Objective The heterotrophic nitrification-aerobic denitrification strains were isolation from the pharmaceutical waste water activate sludge.Methods The strains were obtained through enrichment and cultivation and BTB medium rapid screening.In this paper,we described the taxonomic status of three strains through morphological observation,physiological and biochemical tests,and 16S rRNA sequence.The performance nitrogen removal on aerobic condition was studied.Results PWAS17,PWAS21 and PWAS34 were obtained.PWAS17 was identified as Alcaligenes aquatilis,PWAS21 was identified as Bacillus cereus,and PWAS34 was identified as Pseudomonas sihuiensis.Alcaligenes aquatilis and Pseudomonas sihuiensis was rarely reported.Study on nitrogen removal performanceof three strains was tested.The results indicated that three strains can remove NH-N and COD at the same time.Moreover almost no NO-N or NO-N was accumulated.Experiments indicated that the nitrogen removal rates of PWAS17,PWAS21 and PWAS34 were 92.1%,88.7%and 92.1%when pH 9.0,0.8%sodium citrate as carbon source,5%Amount of inoculation,220 r/min and 30℃.And the basic NH-N concentration in the range of 50~500 mg/L did not inhibit the NH-N removal ability.