火炭母水煎液对金黄色葡萄球菌的抑菌活性

Antibacterial Activity of Water Decoction of Polygonum Chinense L. against Staphylococcus aureus

为了研究火炭母水煎液对金黄色葡萄球菌(S.aureus)的体内外抑菌活性,本试验采用传统水煎法制备火炭母水煎液,分别用牛津杯法和微量肉汤稀释法测定火炭母水煎液对金黄色葡萄球菌的抑菌圈直径、最小抑菌浓度(MIC)和最小杀菌浓度(MBC),通过建立秀丽隐杆线虫——金黄色葡萄球菌感染模型,测定不同浓度火炭母水煎液对金黄色葡萄球菌感染线虫的治疗作用。结果显示,火炭母水煎液浓度为1 000 mg/mL时,对金黄色葡萄球菌的抑菌圈直径为(17.5±0.1)mm, MIC值为15.63 mg/mL,MBC值为31.25 mg/mL;在800~1 000 mg/mL浓度下,火炭母水煎液对金黄色葡萄球菌感染线虫具有较好的治疗作用。结果表明,火炭母水煎液对金黄色葡萄球菌具有良好的体内外抑菌活性,为发现新的抗金黄色葡萄球菌的药物提供理论依据。

This study investigated the bacteriostasis effect of the water decoction of Polygonum Chinense L. on Staphylococcus aureus in vitro and in vivo. Traditional water decocting method was used to prepare water decoction of Polygonum Chinense L. The inhibition zone diameter was evaluated by the Oxford cup method. The minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) were detected by the broth microdilution method. The therapeutic effect of different water decoction concentrations on the model of Caenorhabditis elegans infected with S. aureus was examined. The results showed that with 1 000 mg/mL water decoction of Polygonum Chinense L.,the diameter of inhibition zone against S. aureus was(17.5±0.1) mm, MIC and MBC were 15.63 mg/mL and 31.25 mg/mL, respectively. The water decoction of Polygonum Chinense L. within the concentration range of(800-1 000 mg/mL) had a good therapeutic effect on C. elegans infected with S. aureus. The results indicated that the water decoction of Polygonum Chinense L. exhibited a good antibacterial activity against S. aureus in vivo and in vitro,and forms a basis for the further research on the discovery of new drugs against S. aureus.