负载肉桂醛的气体辅助静电纺丝纳米纤维制备及性质探究

Fabrication and characterization of gelatin/zein/cinnamaldehyde nanofibers by air-assisted electrospinning

利用气体辅助静电纺丝技术制备负载0%、0.1%、0.5%、1.0%(质量分数)肉桂醛的明胶/玉米醇溶蛋白纳米纤维。通过扫描电子显微镜、傅里叶红外光谱和热力学分析探究纳米纤维微观变化。通过机械性能,水蒸气透过率以及抗氧化、抗菌揭示宏观性质。结果表明,采用气体辅助静电纺丝技术可将天然高分子纳米纤维的产率提升至传统方式的10倍,给料速率从传统的1.0 mL/h提升至10.0 mL/h。肉桂醛/明胶/玉米醇溶蛋白纳米纤维直径随肉桂醛添加量的增加无显著性变化,纤维分布均匀。红外光谱分析表明,肉桂醛与蛋白通过氢键相互作用。负载肉桂醛的纳米纤维水蒸气透过率均显著低于明胶/玉米醇溶蛋白纳米纤维,说明负载疏水性的肉桂醛能抑制水分子透过纳米纤维膜。拉伸测试表明,肉桂醛的添加对纳米纤维的断裂伸长率有显著增加,但是对其拉伸强度和杨氏模量无显著影响。抗氧化能力评估发现,1.0%肉桂醛纳米纤维对Fe^(3+)具有较强还原能力。通过抑菌圈法分析发现,肉桂醛/明胶/玉米醇溶蛋白纳米纤维对大肠杆菌和金黄色葡萄球菌具有明显抑制作用。

In this study,gelatin/zein nanofibers loaded with 0%,0.1%,0.5%,and 1.0%(mass ratio) cinnamaldehyde were fabricated by air-assisted electrospinning technique.Investigations of microscopic changes in nanofibers were studied by scanning electron microscopy(SEM) observation,Fourier transform infrared spectroscopy(FTIR) and thermal analysis.Macroscopic properties were revealed by assessing the mechanical property,water vapor permeability,and antioxidant and antibacterial activities.The results showed that compared with the traditional electrospinning technology,the yield of nanofibers increased to ten times through the air-assisted electrospinning technique,and the feeding rate increased from 1.0 mL/h to 10.0 mL/h.The diameters of cinnamaldehyde/gelatin/zein nanofibers showed that there was no significant effect with the increase of cinnamaldehyde,and nanofibers were evenly distributed.FTIR spectra showed that cinnamaldehyde interacted with protein through hydrogen bonding.Nanofibers encapsulated with 0.5% cinnamaldehyde showed better barrier properties against water vapor than the cinnamaldehyde-free nanofibers,indicating that the hydrophobic cinnamaldehyde inhibited water molecules from passing through the nanofibers.Tensile tests showed that the addition of cinnamaldehyde significantly increased the elongation at a break of the nanofibers,but had no significant effect on the tensile strength and elastic modulus.The antioxidant capacity assessment revealed that 1.0% cinnamaldehyde nanofibers had a strong reducing ability for Fe^(3+).The gelatin/zein encapsulated with cinnamaldehyde showed the effective antimicrobial ability of Escherichia coli and Staphylococcus aureus as indicated by the disc diffusion method.