喉癌组织中SEMA3F与NRP2的表达及临床价值

Expression and clinical value of SEMA3F and NRP2 in laryngeal carcinoma

目的探讨喉癌组织中神经轴突导向因子(SEMA3F)、神经菌毛蛋白2(NRP2)的表达及临床意义。方法选取2016年3月~2018年3月我院收治的82例喉癌患者为研究对象,用实时定量PCR法检测癌及癌旁组织中SEMA3F、NRP2 mRNA的表达。以癌组织中SEMA3F、NRP2 mRNA表达的平均数为界,将研究对象分别分为SEMA3F高、低表达组及NRP2高、低表达组。分析SEMA3F、NRP2的表达与临床病理特征关系。Pearson线性相关分析SEMA3F与NRP2表达的相关性。Kaplan-Meier生存分析(Log-Rank检验)癌组织不同SEMA3F、NRP2表达与喉癌患者的生存预后差异。多因素COX回归分析影响喉癌患者生存预后的危险因素。结果与癌旁组织相比,癌组织SEMA3F mRNA表达明显降低,NRP2 mRNA表达明显升高(均P<0.05)。喉癌组织中SEMA3F与NRP2的表达呈显著负相关(r=-0.561,P<0.001)。SEMA3F、NRP2 mRNA的表达与肿瘤临床分期及淋巴结转移有关(均P<0.05)。生存分析结果表明,喉癌组织中SEMA3F低表达、NRP2高表达喉癌患者3年总体生存率明显较差(均P<0.05)。多因素COX回归分析结果喉癌组织中SEMA3F低表达、NRP2高表达、肿瘤Ⅲ~Ⅳ期及淋巴结转移是喉癌患者不良预后的危险因素。结论喉癌中SEMA3F表达降低,NRP2表达增高,均与肿瘤分期及淋巴结转移有关,可能是喉癌预后新的标志物。

Objective To study the expression and clinical significance of nerve axon guidance factor(SEMA3 F)and neuropilin 2(NRP2)in laryngeal carcinoma tissues.Methods A total of 82 patients with laryngeal cancer admitted to our hospital from March 2016 to March 2018 were selected as the research objects.Real-time quantitative PCR was used to detect the expression of SEMA3 F and NRP2 in cancer and adjacent tissues.Taking the mean expression of SEMA3 F and NRP2 mRNA in cancer tissues as the boundary,the subjects were divided into SEMA3 F high and low expression groups or NRP2 high and low expression groups.The relationship between the expression of SEMA3 F,NRP2 and clinicopathological characteristics were analyzed statistically.The correlation between the expression of SEMA3 F and NRP2 were analyzed by Pearson linear correlation analysis.The relationship of SEMA3 F,NRP2 expressions with the survival and prognosis of patients with laryngeal cancer were analyzed by Kaplan-Meier survival analysis(Log-Rank test).The risk factors affecting the survival and prognosis of patients with laryngeal cancer were analyzed by multivariate COX regression analysis.Results Compared with the adjacent tissues,the expression of SEMA3 F mRNA in cancer tissues was significantly lower,while the expression of NRP2 mRNA was significantly higher(all P<0.05).The expression of SEMA3 F and NRP2 in laryngeal cancer tissue was significantly negatively correlated(r=-0.561,P<0.001).The expression of SEMA3 F and NRP2 mRNA is related to the clinical stage of tumor and lymph node metastasis(all P<0.05).The results of survival analysis showed that the 3-year overall survival rate of patients with laryngeal carcinoma with low expression of SEMA3 F mRNA and high expression of NRP2 mRNA in laryngeal carcinoma tissues was significantly poor(both P<0.05).Multivariate Cox regression analysis showed that low expression of SEMA3 F,high expression of NRP2,tumor stageⅢ-Ⅳand lymph node metastasis in laryngeal cancer tissues are risk factors for poor prognosis in patients with laryngeal cancer.Conclusion The expression of SEMA3 F is decreased in laryngeal cancer,while the expression of NRP2 is increased,and both are related to tumor stage and lymph node metastasis,which may be a new prognostic marker of laryngeal cancer.