基于GC-MS的野生和‘鲁赫’刺蔷薇叶片代谢差异研究

Differences in Leaf Metabolism of Wild Rosa acicularis and Rosa acicularis ’Luhe’ Based on GC-MS

为探究野生刺蔷薇(Rosa acicularis)和‘鲁赫’刺蔷薇(Rosa acicularis‘Luhe’)叶片的代谢物和代谢通路差异,对2种刺蔷薇叶片进行了GC-MS非靶向代谢组学试验,共检测出6种氨基酸、12种糖类、4种烷烃类化合物、17种有机酸、5种酯类和7种其他类化合物,共计51种代谢物。PCA模型符合预期,OPLS-DA模型筛选出19种显著差异代谢物,差异代谢通路分析共分析出5条主要的差异代谢通路。综合各项结果,2种刺蔷薇叶片在部分有机酸和糖类的代谢上有显著差异,同时也在ABC转运蛋白、苯丙烷的生物合成、苯甲酸的降解和丙氨酸、天冬氨酸和谷氨酸的代谢上有显著差异,推测2种植物可能在叶片光合效率和抗胁迫能力上有显著不同,但仍需进一步结合基因组、蛋白质组、转录组和其他方法进行深入研究。

In order to explore the differences in the metabolites and metabolic pathways of leaves from wild Rosa acicularis and R. acicularis‘Luhe’,a GC-MS non-targeted metabolomics test was carried out on the leaves of twokinds of R. acicularis,6 amino acids,12 sugars,four alkanes,17 organic acids,five esters and seven othercompounds and a total of 51 metabolites were detected respectively. The PCA model was in line withexpectations and the OPLS-DA model screened out 19 significantly different metabolites. The analysis ofdifferential metabolic pathways revealed a total of five major differential metabolic pathways. Based on theresults,there were significant differences in the metabolism of some organic acids and sugars in as well as in theABC transporter,phenylpropane biosynthesis,benzoic acid degradation and the metabolism of alanine,aspartic acid and glutamic acid in the leaves of the two species of R. acicularis. It was speculated that there weresignificant differences in leaf photosynthetic efficiency and resistance to stress,but further study was needed incombination with genome,proteome,transcriptome and other methods.