灵武长枣果实发育过程中阿拉伯半乳糖蛋白组织化学分布

Histochemical Localization of Arabinogalactan Proteins During Fruit Development from Ziziphus jujuba ’Lingwu Changzao’

为揭示灵武长枣(Ziziphus jujuba ‘Lingwu Changzao’)果实发育过程中阿拉伯半乳糖蛋白(AGPs)的分布规律,以膨大前期、快速膨大期、着色期和完熟期灵武长枣果实为试验材料,通过组织化学和免疫荧光定位的方法,研究了不同发育时期果实AGPs的分布特征。结果显示:βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原在各时期果实的外果皮及相邻的内部数层排列紧密的中果皮小细胞的细胞壁和细胞内部均有分布。中果皮大型卵圆形薄壁细胞的细胞壁和细胞内在膨大前期均有βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布,而在快速膨大期、着色期和完熟期均主要分布于薄壁细胞的细胞壁上,大部分细胞内部无分布;随着果实发育成熟,中果皮薄壁细胞间隙拉大排列更加松散,出现细胞破裂,βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布逐渐减少。各时期果实维管束中维管束鞘、木质部、韧皮部、形成层的所有细胞的细胞壁和细胞内部都分布有βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原,维管束数量和大小随果实发育及体积的进一步增大逐渐减少,βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原分布逐渐减少。研究认为,βGlcY和MAC204分别为研究灵武长枣果实AGPs组化定位有效方法和免疫荧光的良好抗体,各时期果实的不同组织βGlcY-AGPs形成的棕红色沉淀和MAC204抗体识别的抗原荧光强弱存在一定的差异;AGPs可能参与了灵武长枣果实维管束发育过程的形态建成;为果实发育提供保护和营养支持及多糖物质积累。

To reveal the distribution of Arabinogalactan proteins(AGPs)of Ziziphus jujuba ’Lingwu Changzao’ during fruit development,fruits in the early bulking period,rapid enlargement period,coloring period and maturation period were used as materials and the distribution of AGPs were identified by methods of histochemistry and immunofluorescence localization respectively. The results showed the brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of the epicarp and the alongside internal layers of closely arranged mesocarp small cells in different periods fruit. The brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and the inner part of the large ovoid parenchyma cells in the inner mesocarp during the early bulking period,while they were mainly distributed in the parenchyma cell walls during the rapid enlargement period,coloring period and maturation periods,with no distribution in the inner part of most cells.With the ripening of fruits,the mesocarp parenchyma cells became more loosely arranged with elongated intercellular spaces,cell rupture occurred,and the distribution of brown red precipitate formed by βGlcYAGPs and antigen recognized by MAC204 antibody gradually decreased. The brown red precipitate formed byβGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of all cells in the vascular bundle sheaths,xylem,phloem,and cambium of vascular bundles in different periods fruit respectively,and the number and size of vascular bundles gradually decreased with fruit development and further increased in size,and the distribution of brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody gradually decreased. The results suggested that βGlcY and MAC204 were respectively effective method for histochemical localization and good antibody for immunofluorescence of Z.jujuba‘Lingwu Changzao’fruit,and there were some differences in brown red precipitate formed by βGlcY and the fluorescence intensity of antigen recognized by MAC204 antibody in different tissues of fruits at different periods,AGPs probably participated in morphogenesis during vascular bundles development of Z. jujuba‘Lingwu Changzao’fruit,which provided protection and nutritional support and polysaccharide accumulation for fruit development.