Caco-2细胞模型评价食品功能因子的吸收、代谢及其功能研究进展

Research Progress of Evaluating the Absorption, Metabolism and Function of Food Functional Factors Using the Caco-2 Cell Model

Caco-2细胞源自于人结肠癌细胞系,其表现出许多与小肠上皮细胞相似的特性,具有微绒毛结构、刷状边缘以及细胞间紧密连接等,是目前应用最广泛、最经典的模型之一。近年来,随着细胞培养技术的发展,通过体外细胞培养来评价食品功能因子的吸收、代谢机制及其功能成为研究热点。本文从Caco-2细胞模型的建立和评估方法入手,对功能食品提取物在Caco-2细胞中的抗炎、抗氧化、抗增殖等功能性进行系统概述,再结合功能食品提取物在Caco-2细胞中的吸收、代谢、转运机制,对Caco-2细胞模型在共培养等方面的研究进行展望,以期改进Caco-2细胞模型的局限性,为进一步研发准确度高,材料成本低,更接近人体内部环境的细胞培养模型奠定基础。

Caco-2 cells are derived from human colon cancer cell lines, which exhibit many characteristics similar to small intestinal epithelial cells like microvilli structure, brush-like edges, and tight junctions. It is currently the most widely used and classic model about test in vitro. In recent years, with the development of cell culture technology. The evaluation of the absorption and metabolism mechanism and function of food functional factors by cell model which has become a research hotspot. This article starts with the establishment and evaluation methods of the Caco-2 cell model and systematically summarizes the anti-inflammatory, anti-oxidation and anti-proliferation of functional food extracts in Caco-2 cells. Then absorption, metabolism, and transport mechanisms in cells are combined to provide a prospect for the development of the Caco-2 cell model in the field of co-culture. In order to improve the limitations of the Caco-2 cell model and lay the foundation for the further development of a cell culture model with high accuracy, high cost-effectiveness and closer to the internal environment.