circERBB2靶向miR-2682-5p调控肝癌HCCLM3细胞生物学行为

CircERBB2 targets miR-2682-5p to regulate the biological behavior of liver cancer HCCLM3 cells

目的探讨环状RNA(circular RNA,circRNA)circERBB2是否通过靶向miR-2682-5p调控肝癌HCCLM3细胞生物学行为。方法应用qRT-PCR检测45例肝癌组织中circERBB2和miR-2682-5p表达。HCCLM3细胞分为si-NC组、si-circERBB2组、miR-NC组、miR-2682-5p组、si-circERBB2+anti-miR-NC组、si-circERBB2+anti-miR-2682-5p组。应用CCK-8、克隆形成测定、划痕愈合实验、Transwell实验检测细胞活力、克隆形成、迁移和侵袭。双荧光素酶报告实验验证circERBB2和miR-2682-5p的靶向结合。结果与癌旁组织比较,肝癌组织中circERBB2表达显著升高(P<0.05),miR-2682-5p表达显著降低(P<0.05)。si-circERBB2组HCCLM3细胞活力、克隆形成数、划痕愈合率、侵袭细胞数显著低于si-NC组(P<0.05)。miR-2682-5p组HCCLM3细胞活力、克隆形成数、划痕愈合率、侵袭细胞数显著低于miR-NC组(P<0.05)。circERBB2靶向调控miR-2682-5p的表达。si-circERBB2+anti-miR-2682-5p组HCCLM3细胞活力、克隆形成数、划痕愈合率、侵袭细胞数显著高于si-circERBB2+anti-miR-NC组(P<0.05)。结论干扰circERBB2表达通过靶向miR-2682-5p,对肝癌HCCLM3细胞增殖、迁移和侵袭显示出抑制作用。

Objective To investigate whether circular RNA(circRNA)circERBB2 regulates liver cancer progression by targeting miR-2682-5 p.Methods qRT-PCR was used to detect the expression of circERBB2 and miR-2682-5 p in 45 cases of liver cancer tissues.Liver cancer HCCLM3 cells were divided into si-NC group,si-circERBB2 group,miR-NC group,miR-2682-5 p group,si-circERBB2+anti-miR-NC group,si-circERBB2+anti-miR-2682-5 p group.CCK-8 and colony formation,scratch healing test and Transwell test were applied to determine the cell viability,clone formation,migration and invasion.The dual luciferase reporter experiment verified the targeted binding of circERBB2 and miR-2682-5 p.Results Compared with adjacent tissues,the expression of circERBB2 in liver cancer tissue was significantly increased(P<0.05),while the expression of miR-2682-5 p was significantly decreased(P<0.05).The cell viability,clone formation number,scratch healing rate,invasive number of HCCLM3 cells in the si-circERBB2 group were notably lower than those in the si-NC group(P<0.05).The cell viability,clone formation number,scratch healing rate,invasive number cells of HCCLM3 cells in miR-2682-5 p group were notably lower than those in the miR-NC group(P<0.05).circERBB2 targeted and regulateed the expression of miR-2682-5 p.The cell viability,clone formation number,scratch healing rate,invasive number of HCCLM3 cells in the si-circERBB2+anti-miR-2682-5 p group were notably higher than those in the si-circERBB2+anti-miR-NC group(P<0.05).Conclusion Interfering the expression of circERBB2 by targeting miR-2682-5 p can inhibit the proliferation,migration and invasion of HCCLM3 cells.