^(60)Co-γ射线辐射对豫谷18种子萌发及幼苗生长的影响

Effects of ^(60)Co-γ Ray Radiation on Germination and Seedling Growth of Yugu 18

为了研究不同剂量^(60)Co-γ射线对谷子种子的辐射诱变效应,以豫谷18为材料,采用0,150,200,250,300和350 Gy的^(60)Co-γ射线辐射谷子种子,探究辐射对谷子种子萌发、幼苗生长及生理生化指标的影响。结果表明,豫谷18在低辐射剂量(150~200 Gy)下对种子发芽、出苗有促进作用,随着辐射剂量升高种子萌发及幼苗生长逐渐受到抑制,350 Gy剂量下对谷子种子损伤较大,严重影响其出苗及幼苗生长,幼苗成苗率急剧降低;随着辐射剂量增加,谷子叶片中SOD活性、POD活性整体呈逐渐上升趋势,SPAD值呈先升高后下降的变化趋势。根据半致死剂量回归方程计算出豫谷18的半致死剂量为331.7 Gy。

In order to study the radiation mutagenic effect of different doses of ^(60)Co-dγrays on foxtail millet seeds,Yugu 18 was used as experimental material.Foxtail millet seeds were irradiated with ^(60)Co-γrays of 0,150,200,250,300 and 350 Gy to explore the effects of radiation on seed germination,seedling growth and physiological and biochemical indexes of foxtail millet.The results showed that Yugu 18 could significantly promote seed germination and seedling emergence at low radiation dose(150-200 Gy).With the increase of radiation dose,seed germination and seedling growth are gradually inhibited,while 350 Gy dose will seriously damage foxtail millet seeds,seriously affect their seedling emergence and seedling growth,and the seedling emergence rate will drop sharply;With the increase of radiation dose,SOD activity and POD activity content in foxtail millet leaves showed a gradual upward trend,and SPAD value increased first and then decreased.According to the half lethal dose regression equation,the half lethal dose of Yugu 18 is 331.7 Gy.