凡口铅锌矿废弃地植物促生长内生真菌的筛选

Screening of Growth Promoting Endophytic Fungi in Abandoned Fankou Lead-Zinc Mine

以粤北凡口铅锌矿废弃地优势植物香蒲(Typhaorientalis presl)、苦楝(Melia azedarach)、苎麻(Boehmeria nivea)和蓖麻(Ricinus communis)中分离筛选出的具有抗重金属铅、锌能力的45株内生真菌为研究材料,利用CAS检测液显色反应、钼锑抗比色法和Salkowski法,分别筛选出具有促进植物生长的产铁载体菌株30株、溶磷菌株3株和产吲哚乙酸(IAA)菌株18株。并选取具有产铁载体、溶磷或产IAA能力较强的菌株作为代表菌株进行形态学鉴定和ITS序列分析,鉴定结果显示:WB-21为曲霉属(Aspergillus)、BM3为旋孢腔菌属(Cochliobolus)、BM5为链格孢属(Alternaria)、菌株XP3.21为刺盘孢属(Colletotrichum)、ZMP5-3为篮状菌属(Talaromyces)。

With 45 endophytic fungi with the ability to resist heavy metal lead and zinc isolated from the dominant plants of Typhaorientalis presl,Melia azedarach,Boehmeria nivea and Ricinus communis in the Fankou lead and zinc mine wasteland in northern Guangdong as the research target,30 iron producing carrier strains,3 phosphorus solubilizing strains and 18 indoleacetic acid(IAA)producing strains,all with the ability to promote plant growth,are screened by using CAS detection solution color reaction,molybdenum antimony resistance colorimetry and Salkowski method,followed by a selection of the isolates with strong ability of producing iron carrier,dissolving phosphorus or producing indole acetic acid(IAA)as representative isolates for identification of morphological and ITS sequence analysis.The identification results show that WB-21 is under the category of Aspergillus,BM3 Cochliobolus,BM5 Alternaria,XP3.21 Colletotrichum,and ZMP5-3 Talaromyces respectively.