寡雄腐霉与氟噻唑吡乙酮协同防治烟草黑胫病研究

Synergistic Control Effect of Oxathiapiprolin and Pythium oligandrum Against Tobacco Black Shank

为了探究寡雄腐霉(Pythium oligandrum,PO)与氟噻唑吡乙酮(Oxathiapiprolin,OX)联合防控烟草黑胫病的可行性,采用菌丝生长速率法和平板对峙法分别测定了PO和OX的生物相容性,及OX、PO和OX+PO对黑胫病菌的毒力,并通过盆栽试验分别测定了OX、PO和OX+PO对烟草黑胫病菌的防效,以及对烟草幼苗的促生效应。生物相容性试验结果表明,不同浓度OX处理下,PO的生长速率与空白对照无显著差异。室内毒力结果显示,8.0×10^(-3)mg/L OX对烟草黑胫病菌的抑制率为91%,EC为2.19×10^(-3)mg/L,而OX(1.0×10^(-3)mg/L)与PO联用的抑制率达96.31%。盆栽试验结果表明,OX+PO处理对烟草黑胫病的防效最高(84.57%),比OX(77.76%)或PO(76.54%)单独处理防效分别提高8.76%和10.49%。OX和PO联用对烟草促生效果显著,其株高、茎粗、叶长、叶宽分别比对照处理提高了57.10%、20.21%、33.62%、35.08%。以上结果表明,PO与OX联用能够有效降低OX的使用量,提高防病效果,促进烟草生长。

To explore the potential of the synergism of Pythium oligandrum(PO)combined with Oxathiapiprolin(OX)against tobacco black shank,the biocompatibility of PO and OX,as well as the toxicity of OX,PO and OX+PO against Phytophthora nicotianae were measured by the growth rate method and flat confrontation method,while the synergistic antifungal effect and the growth promotion of tobacco were determined in vivo.The biocompatibility test showed that the growth rate of PO with different concentrations of OX treatments has no significant difference from that of the blank control.The results of in vitro toxicity test indicated that the inhibition rate of OX at 8.0×10^(-3)mg/L on P.nicotianae was 91%,EC=2.19×10^(-3)mg/L,while that of OX(1.0×10^(-3)mg/L)+PO was 96.31%.The results of pot experiment showed that the control effect of the OX+PO treatment was the highest(84.57%),which was 8.76%and 10.49%higher than that of OX(77.76%)or PO(76.54%)treatment alone,respectively.The combined use of OX and PO had a significant promotion on tobacco growth,and the plant height,stem diameter,leaf length and leaf width were increased by 57.10%,20.21%,33.62%and 35.08%,respectively,compared with the control treatment.The above results indicated that the combined use of PO and OX can effectively reduce the amount of OX used,improve the disease prevention effect,and promote the growth of tobacco.