外施GA_(3)对茉莉花主要萜类物质及其相关基因表达的影响

Changes of Major Terpenoids Contents and Related Genes’Expression Level in Jasmine after GA_(3)Treatment

对茉莉花喷施100μmol·L^(–1)赤霉素(GA_(3)),分析赤霉素对茉莉花主要香气组分及其合成相关结构基因和转录因子表达水平的影响。结果显示,外施GA_(3)可提高花朵开放过程中总香气成分以及芳樟醇、法呢烯含量,与此同时,花朵开放过程中总香气物成分、法呢烯含量高峰提前;外施GA_(3)使花朵开放过程中JsTPS2、JsTPS3、JsTPS4基因表达峰值提高,JsTPS1基因的表达水平降低;外施GA_(3)后,在花朵开放过程中花香相关转录因子JsMYB305、JsMYB86、JsMYB108的表达水平提高,JsMYC2的表达水平降低,而基因JsbHLH13的表达水平提高。研究结果可为施加植物生长调节物质提高茉莉花香气提供理论依据。

In this study,100μmol·L^(–1)gibberellin(GA_(3))was used to spray jasmine,and its effects on the main aroma components of jasmine and the expression levels of aroma synthesis-related structural genes and transcription factors were analyzed.The results showed that external GA_(3)could increase the total aroma components and the contents of linalool and farnesene during the blooming process,and make the peaks of total aroma components and farnesene content earlier during the blooming process.GA_(3)treatment increased the expression peaks of JsTPS2,JsTPS3,and JsTPS4 genes during flower opening,while the expression level of JsTPS1 decreased.After external application of GA_(3),the expression levels of floral aroma-related transcription factors JsMYB305,JsMYB86,and JsMYB108 increased during flower opening.The expression level of JsMYC2 decreased during flower opening,while the expression level of JsbHLH13 increased.The research results could provide a theoretical reference for improving the aroma of jasmine through plant growth regulators.