海马内注射鸢尾素缓解小鼠抑郁样行为

Intrahippocampal injection of irisin ameliorates depression-like behaviors in mice

目的探讨鸢尾素对抑郁症模型小鼠抑郁样行为的影响。方法将C57BL/6小鼠随机分成模型组和健康组,模型组采用开放空间强迫游泳(OSFS)方法建立抑郁小鼠模型,造模成功后将造模组随机分成“模型-对照”组和“模型-鸢尾素”组。健康野生小鼠正常饲养,同样随机分为“健康-对照”组和“健康-鸢尾素”组。所有鸢尾素组小鼠通过脑立体定位注射向两侧海马分别注射77 pmol鸢尾素,对照组注射同体积的溶剂(PBS)。利用强迫游泳及悬尾检测等实验对其抑郁样行为进行检测。结果与健康组比较,抑郁模型组小鼠在强迫游泳测试(P<0.01)及悬尾测试(P<0.05)中的不动时间显著延长,证明成功模拟了抑郁症患者的绝望样行为,模型构建成功。与“模型-对照”组相比,鸢尾素可显著缩短“模型-鸢尾素”组小鼠在强迫游泳实验(P<0.001)及悬尾实验(P<0.05)中的不动时间。结论鸢尾素可显著改善开放空间强迫游泳抑郁模型小鼠的抑郁样行为。

Objective To investigate the effects of irisin on the depression-like behaviors in depression mice.Methods C57BL/6 mice were randomly divided into model and healthy groups.Mice in the model group were trained by open-space forced swim(OSFS)model and then were sub-divided into“model-control”and“model-irisin”.Healthy wild mice were bred normally and sub-divided into“healthy-control”and“healthy-irisin”groups.All mice in test group and in control groups were injected with 77 pmol irisin or the solvent(PBS)into both hippocampi by brain stereotactic injection,respectively.Depression-like behaviors were examined by forced swim test(FST)and tail suspension test(TST).Results Compared with the healthy ones,the model mice showed a markedly prolonged immobility time in FST(P<0.01)and TST(P<0.05),which indicated that the successfully established model can simulate the despair-like behaviors of patients with depression.Compared with the“model-control”,irisin can remarkably reduce the immobility time of“model-irisin”mice in FST(P<0.001)and TST(P<0.05).Conclusions Irisin can significantly improve the depression-like behaviors of OSFS depression mice.