一株益生型枯草芽孢杆菌液态发酵条件优化

Optimization of Liquid Fermentation Conditions of a Probiotic Bacillus subtilis Strain

本研究通过实验优化了一株有改善肠道功能的枯草芽孢杆菌的液态发酵工艺。优化培养基为0.5%葡萄糖、2%大豆蛋白胨、MgSO_(4)·7H_(2)O(0.07%)、MnSO_(4)·H_(2)O(0.03%)、KH_(2)PO_(4)(0.2%)、K_(2)HPO_(4)(0.2%),采用补料分段发酵,发酵液活菌由原始1.5×10^(8) CFU·mL^(-1)提高到8.4×10^(9) CFU·mL^(-1),优化效果显著。

In this study,the liquid fermentation process of a strain of Bacillus subtilis with improved intestinal function was optimized through experiments.The optimized medium was 0.5%glucose,2%soy peptone,MgSO_(4)·7H_(2)O(0.07%),MnSO_(4)·H_(2)O(0.03%),KH_(2)PO_(4)(0.2%),K_(2)HPO_(4)(0.2%),and the fermentation broth was fermented in stages.The viable bacteria increased from the original 1.5×10^(8) CFU·mL^(-1) to 8.4×10^(9) CFU·mL^(-1),and the optimization effect was remarkable.