酱香大曲中高温放线菌筛选方法研究

Study on the Screening Method of Thermoactinomycetes in Maotai-flavored Daqu

经过四种样品预处理方式,采用适宜的稀释剂、不同的抑制剂、四个温度梯度培养大曲中的高温放线菌,最后分离筛选出了6株高温放线菌,使用该大曲作为样品其中最高效的分离筛选组合方式是利用CaCO_(3)富集培养3天的样品预处理方法,添加40 mg/L制霉菌素和60 mg/L新生霉素作为抑制剂,利用GTY培养基在55℃下培养3~4 d。该分离筛选方法为后续高温放线菌的鉴定等实验奠定了基础,尽可能填补高温放线菌分离筛选及其功能探究的空白。

In this paper, after four sample pretreatment methods, appropriate diluents, different inhibitors, and four temperature gradients were used to cultivate the high-temperature actinomycetes in Daqu. Finally, 6 strains of Thermoactinomycetes were isolated and screened. As a sample, the most efficient separation and screening combination method is to use the sample pretreatment method of CaCO_(3) enrichment culture for 3 days, adding 40 mg/L nystatin and 60 mg/L novobiocin as inhibitors, using GTY medium at 55℃ Culture for 3 to 4 days. This isolation and screening method lays the foundation for subsequent experiments such as the identification of Thermoactinomycetes, and fills the gap in the isolation, screening and functional exploration of Thermoactinomycetes as much as possible.