响应面法优化南极磷虾蛋白肽脱色工艺

Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology

为优化南极磷虾蛋白肽脱色工艺,提高南极磷虾蛋白肽产品品质,采用活性炭吸附法脱除南极磷虾蛋白肽溶液中的色素,以脱色率和蛋白保留率为评价指标,分别考察活性炭用量、pH、脱色温度、脱色时间对脱色效果的影响,在单因素实验基础上,选择脱色温度50℃,采用响应面法优化南极磷虾蛋白肽脱色工艺。结果表明,采用粉末活性炭吸附脱除南极磷虾蛋白肽色素的最佳条件为:活性炭用量4.0%、pH1.5、脱色时间1.0 h;在此条件下,脱色率达到82.19%±0.20%,蛋白保留率为90.93%±2.28%。采用优化工艺对南极磷虾蛋白肽进行脱色处理,样品氨基酸组成中必需氨基酸与非必需氨基酸的占比以及样品的分子量分布不会发生明显变化。研究将为优质南极磷虾蛋白肽产品开发提供支撑。

In order to optimize the decolorization process of Antarctic krill peptides and improve the product quality,activated carbon adsorption method was used to remove the pigment from the Antarctic krill peptides solution.Taking the decolorization rate and protein retention rate as the evaluation indexes,the influence of four independent variables including dosage of activated carbon,pH,decolorization temperature and decolorization time on the decolorization effect were investigated respectively.On the basis of single factor experiment,three independent variables including dosage of activated carbon,pH and decolorization time were optimized by the response surface methodology under the decolorization temperature of 50℃.Results showed that powdered activated carbon was more suitable for the removal of pigment from Antarctic krill peptides when compared with granular activated carbon,and the optimal conditions determined by response surface methodology were as follows:dosage of activated carbon 4.0%,pH1.5 and decolorization time 1.0 h.Under the optimal conditions,the decolorization rate was 82.19%±0.20%and the protein retention rate was 90.93%±2.28%.The decolorization treatment had no obvious effects on the proportion of essential amino acids and nonessential amino acids in the amino acid compositions as well as the molecular weight distribution of Antarctic krill peptides.This research would provide scientific support for the development of high-quality Antarctic krill peptides products.