4-巯基苯硼酸功能化的金纳米溶胶用于细菌的快速SERS检测

4-Mercaptophenylboronic Acid Functionalized Gold Nanosol for Rapid SERS Detection of Bacteria

本文采用经典柠檬酸钠还原法制备金纳米粒子(AuNPs)溶胶作为SERS基底,使用4-巯基苯硼酸(4-MPBA)作为拉曼报告分子以及细菌识别元件来功能化AuNPs。基于细菌抑制盐诱导聚集的检测机制,构建了一种广谱细菌的标记SERS检测方法。在优化实验条件下,细菌在10 CFU/mL-10^(5) CFU/mL浓度范围内与4-MPBA@AuNPs的拉曼信号强度有良好的线性关系,定量检测限为10 CFU/mL,加标回收率为89.2%-103.8%,相对标准偏差小于4.7%。本方法灵敏、快速、低成本、操作简单,在实际样品的现场快速检测中具有潜在应用价值。

Fast and accurate detection of pathogenic bacteria is essential to ensure food safety and public health safety.In this paper, the classical sodium citrate reduction method was used to prepare gold nanoparticle(AuNPs) sol as the SERS substrate, and 4-mercaptophenylboronic acid(4-MPBA) was used as the Raman reporter and bacteria recognition element to functionalize AuNPs.Based on the detection mechanism of bacteria inhibiting salt-induced aggregation, a broad-spectrum bacteria labeled SERS detection method was constructed.Under optimized experimental conditions, the amount of bacteria has a good linear relationship with the Raman signal intensity of 4-MPBA@AuNPs within the concentration range of 10 CFU/mL-10^(5) CFU/mL,the quantitative detection limit is 10 CFU/mL,the recovery rate of standard addition is 89.2%-103.8%,and the relative standard deviation is less than 4.7%.The method is sensitive, fast, low-cost, simple to operate, and has potential application value in rapid detection of actual samples.