评价乳腺癌免疫组织化学染色及质量控制的临床效果

To Evaluate the Clinical Effect of Breast Cancer Immunohistochemical Staining and Quality Control

目的分析乳腺癌免疫组织化学染色的临床效果及质量控制的方式。方法选入我院于2017年10月至2018年10月进行治疗的乳腺癌患者50例,将50例患者的病理标本分成两份,一份进行免疫组织化学染色,列为免疫组织化学染色组,另外一份进行常规处理,列为常规组,对比两组不同的染色方式的结果、免疫组织化学染色组的染色结果、两种检测方式的显色结果以及染色质量优良率、染色方式的满意度。结果①免疫组织化学染色诊断准确率优于常规组(90.00%vs.60.00%),组间具有统计学意义(χ^(2)=12.0000,P=0.0005)。②免疫组织化学染色组进行ER、PR、CerbB-2、P53、Ki67检测,阳性率分别为76.00%、56.00%、58.00%、72.00%、80.00%。③常规组染色后较为鲜艳,并且细胞核呈现出较为鲜明的蓝色,细胞质则能够出现深浅不一的粉红色,核仁为红色;免疫组织化学染色组:ER、PR中切片为深褐色,CerbB-2下切片的细胞核为蓝色,细胞膜为褐色。免疫组织化学染色组方案下的染色质量优良率为96.00%,常规组方案下的染色质量优良率为84.00%,免疫组织化学染色组高于常规组,有统计学意义(P<0.05)。免疫组织化学染色组获得的染色情况(98.00%)、染色结果(100.00%)、诊断结果(96.00%)、使用价值满意度(98.00%)均高于常规组(86.00%、90.00%、82.00%、84.00),P<0.05,差异显著(χ^(2)=4.8913、5.2632、5.0051、5.9829,P=0.0270、0.0218、0.0253、0.0144)。结论对乳腺癌进行免疫组织化学染色出现错诊、漏诊的情况较少,通过切片的显色情况进行病理结果的确定,其准确率较高,可为临床治疗方案的制定提供数据支撑,值得在临床上推广和应用。

Objective To analyze the clinical effects and quality control methods of breast cancer immunohistochemical staining.Methods Fifty patients with breast cancer who were treated in our hospital from October 2017 to October 2018 were selected.The pathological specimens of 50 patients were divided into two,and one was subjected to immunohistochemical staining and classified as the immunohistochemical staining group,the other one was treated routinely and was classified as the conventional group.The results of different staining methods of the two groups were compared with the staining results of the immunohistochemical staining group and the coloring results of the two detection methods.Results(1)The diagnostic accuracy of immunohistochemical staining was 90.00%better than that of conventional group 60.00%,with statistical significance between groups(χ^(2)=12.0000,P=0.0005).(2)The immunohistochemical staining group was tested for ER,PR,CerbB-2,P53,Ki67,and the positive rates were 76.00%,56.00%,58.00%,72.00%,and 80.00%,respectively.(3)The conventional group was more vivid after staining,and the nucleus showed a more vivid blue.The cytoplasm was able to appear in different shades of pink,and the nucleoli was red.Immunohistochemical staining group:the sections in ER and PR were dark brown,CerbB-2 the nuclei of sections were blue and the cell membrane was brown.The excellent and good rate of staining quality in the immunohistochemical staining group was 96.00%,and the staining quality in the routine group was 84.00%.The immunohistochemical staining group was higher than the routine group,P<0.05,which was statistically significant.The staining condition(98.00%),staining result(100.00%),diagnosis result(96.00%),and satisfaction degree(98.00%)obtained by the immunohistochemical staining group were higher than those of the routine group(86.00%,90.00%,82.00%,84.00%)(χ^(2)=4.8913,5.2632,5.0051,5.9829,P=0.0270,0.0218,0.0253,0.0144).Conclusion There are fewer cases of misdiagnosis and missed diagnosis of breast cancer with immunohistochemical staining.The pathological results are determined by the color development of the section.The accuracy is high,which can provide data support for the formulation of clinical treatment plans.Clinical promotion and application.