软衬硅橡胶表面仿树蛙足垫微阵列结构对吸附力影响的体外研究

Study on the effect of silicone soft liner with bionic microarray structure mimicking tree frog’s foot pad on adhesion force in vitro

目的制作表面含有不同尺寸仿树蛙足垫微结构的软衬硅橡胶样片,评价其在模拟口腔环境中的吸附力差异,筛选出仿树蛙足垫微结构的最优参数。方法设计4种不同尺寸(六棱柱边长×高度×六棱柱间距:A组100μm×75μm×33μm,B组100μm×75μm×60μm,C组40μm×75μm×75μm,D组40μm×75μm×50μm)的仿树蛙足垫微结构,3D打印出带有反结构的阴模,软衬硅橡胶翻模,得到表面带有仿生微结构的样片。扫描电镜观察4组样片的表面形态,并用电子万能材料试验机在模拟口腔环境中进行吸附力测试。表面光滑的平面样片作为对照组E。结果4个实验组的吸附力分别为A组(0.32±0.02)N、B组(0.28±0.02)N、C组(0.19±0.02)N、D组(0.27±0.02)N,与对照组E的(0.17±0.02)N相比,差异均有统计学意义(P<0.05),B、D组间吸附力差异没有统计学意义(P>0.05),其余任意两组间的吸附力差异均有统计学意义(P<0.05)。其中,A组吸附力最好。结论在软衬硅橡胶材料表面制作仿树蛙足垫微结构可以提高其吸附力,这为改善全口义齿固位力提供了一种新思路,但其临床应用仍待进一步研究。

Objective The study aimed to investigate the difference in adhesion force of silicone soft liner samples with different sizes of micropattern mimicking tree frog’s foot pads in the simulated oral environment and screen out the optimal parameters of bionic tree frog’s foot pads.Methods Four different sizes of tree frog’s foot pad structure were designed(hexagonal pillar side length×height×hexagonal pillar spacing:Group A 100μm×75μm×33μm,Group B100μm×75μm×60μm,group C 40μm×75μm×75μm,group D 40μm×75μm×50μm).Firstly,the negative mold of tree frog’s foot pads with reverse structure was 3D printed,and then the mold was flipped with silicone soft liner to obtain bionic microstructure on the surface.The surface morphology of sample was observed by scanning electron microscopy(SEM),and the adhesion force was tested in the simulated oral environment by electronic universal material testing machine.The plane sample with smooth surface was used as the control group E.Results Statistical analysis of adhesion force data showed that the adhesion force in Group A,Group B,Group C and Group D[(0.32±0.02)N,(0.28±0.02)N,(0.19±0.02)N,(0.27±0.02)N respectively]was significantly different from that of the control group E[(0.17±0.02)N](P<0.05),there was no statistically significant difference in adhesion force between group B and group D(P>0.05),and there were statistically significant differences in adhesion force between the other two groups(P<0.05).Among them,group A had the best adhesion force.Conclusion The results showed that the microstructure mimicking tree frog’s foot pad on the surface of silicone soft liner surface can improve its adhesion force,providing a new idea for improving the retention capacity of complete denture,but its clinical application still needs further study.