环状RNA TADA2A在肝癌组织中的表达及其对肝癌细胞凋亡的影响

Expression of circular RNA TADA2A in hepatocellular carcinoma and its effect on apoptosis of hepatocellular carcinoma cells

目的:探讨环状RNA TADA2A(circTADA2A)在肝癌组织中的表达及其对肝癌细胞凋亡的影响。方法:对海南医学院第二附属医院80名肝癌患者病理采样肝癌病灶及癌旁组织,采用实时定量逆转录PCR法(RT-qPCR)检测circTADA2A的相对表达量,t检验分析统计学差异,并进一步采用χ^(2)检验分析circTADA2A的相对表达量与临床病理特征的关系;顺时转染空白质粒和circTADA2A过表达质粒;Western blot检测人肝癌HepG2细胞凋亡指标Casapase3,t检验分析统计学差异。结果:肝癌组织中的circTADA2A的相对表达量明显高于癌旁组织(2.03±0.12>0.45±0.03,t=10.358,P=0.001),差异有统计学意义(P<0.05);circTADA2A相对表达水平与肿瘤T分期(χ^(2)=7.301,P=0.002)和分化程度(χ^(2)=10.651,P=0.000)呈显著相关,差异有统计学意义(P<0.05);circTADA2A过表达的HepG2细胞凋亡指标Casapase3相对表达量明显低于空白对照HepG2细胞(3.106±0.346<5.552±0.145,t=9.265,P=0.015),差异有统计学意义(P<0.05)。结论:circTADA2A在肝癌组织中高表达,并且与肿瘤T分期及分化程度呈显著相关性,同时可以抑制人肝癌HepG2细胞凋亡,这为临床上肝癌诊断和治疗提供了一个新的思路。

Objective:To investigate the expression of circular RNA TADA2A(circTADA2A)in liver cancer tissues and its effect on apoptosis of liver cancer cells.Methods:The liver cancer lesions and adjacent tissues were pathologically sampled from 80 liver cancer patients in The Second Affiliated Hospital of Hainan Medical College,and the relative expression level of circTADA2A was detected by Reverse transcription-quantitative PCR(RT-qPCR),and the t-test was used to analyze the statistical differences.The relative expression level of circTADA2A was analyzed byχ^(2) test and its relationship with clinicopathological characteristics.The apoptosis index Casapase3 of human hepatocellular carcinoma HepG2 cells was detected by Western blot,and the difference was statistically analyzed by t test.Results:The relative expression level of circTADA2A in liver cancer tissues was significantly higher than that in adjacent tissues(2.03±0.12>0.45±0.03,t=10.358,P=0.001),and the difference was statistically significant(P<0.05).The relative expression level of circCADA2a was significantly correlated with tumor T stage(χ^(2)=7.301,P=0.002)and tumor differentiation degree(χ^(2)=10.651,P=0.000),and the difference was statistically significant(P<0.05).The relative expression level of Casapase3 in circTADA2A overexpressed HepG2 cells was significantly lower than that in blank control HepG2 cells(3.106±0.346<5.552±0.145,t=9.265,P=0.015),and the difference was statistically significant(P<0.05).Conclusion:The high expression of circTADA2A in HCC tissues is significantly correlated with tumor T stage and differentiation degree and can inhibit the apoptosis of human HepG2 cells,which provides a new idea for clinical diagnosis and treatment of HCC.