卵巢恶性囊肿组织中miR-484、微管相关蛋白2的表达及临床意义

Expressions and clinical significance of miR-484 and microtubule-associated protein 2 in ovarian malignant cyst tissue

目的:探讨卵巢恶性囊肿组织中miR-484、微管相关蛋白2(MAP2)表达及与患者预后的关系。方法:回顾性收集2015年6月-2018年6月来本院治疗的卵巢恶性囊肿患者154例为恶性组,一般资料相符行卵巢良性囊肿手术的患者154例为良性组,收集两组患者术中囊肿组织,采用免疫组织化学法和Western blot检测MAP2表达,实时荧光定量PCR(qRT-PCR)法检测miR-484表达;Pearson法分析miR-484与MAP2表达的相关性;利用Kaplan-Meier法分析不同miR-484、MAP2表达水平与患者生存时间的关系;采用多因素Cox回归风险模型分析影响卵巢恶性囊肿患者预后的因素。结果:恶性组MAP2高于良性组,miR-484低于良性组,二者表达呈负相关(r=-0.498)(均P<0.05);卵巢恶性囊肿组织中MAP2、miR-484表达与患者年龄、病理类型、肿瘤直径、浸润程度无关,与分化程度、淋巴结转移、大网膜转移、FIGO分期、组织病理分级有关,差异有统计学意义(均P<0.05);Kaplan-Meier分析显示,MAP2高表达患者3年累积生存率(44.9%)低于低表达患者(65.8%)(Log rankχ^(2)=7.432,P=0.006);miR-484高表达患者3年累积生存率(65.8%)高于低表达组患者(44.0%)(Log rankχ^(2)=8.819,P=0.003)。Cox结果显示,MAP2高表达、miR-484低表达、淋巴结转移、FIGO分期、分化程度MAP2高表达、miR-484低表达、淋巴结转移、FIGO分期、分化程度、大网膜转移均是影响患者预后的危险因素。结论:卵巢恶性囊肿组织中miR-484低表达,MAP2高表达,二者表达负相关且与患者病情发展及预后关系密切。

Objective:To investigate the correlation between the expressions of miR-484 and microtubule-associated protein 2(MAP2)in ovarian malignant cyst tissue of patients and their prognosis.Methods:A total of 154 patients with ovarian malignant cysts from June 2015 to June 2018 were collected in study group retrospectively,and 154 patients with the similar general data who had undergone surgery for benign ovarian cysts were selected in control group.The cyst tissues of the patients in the two groups were collected.The expression of MAP2 in cyst tissues was detected by immunohistochemistry and Western blot,and the expression of miR484 in cyst tissues was detected by real-time fluorescence quantitative PCR(qRT-PCR)method.The correlation between the miR-484 expression and the MAP2 expression was analyzed by Pearson method.Kaplan-Meier method was used to analyze the relationship between the expression levels of different miR-484 and MAP2 of the patients and their survival time.Multivariate Cox regression risk model was used to analyze the prognostic factors of the patients with ovarian malignant cysts.Results:The expression of MAP2 of the patients in the study group was significantly higher than that of the patients in the control group,and the expression of miR-484 was of the patients in the study group was significantly lower,and the expression of MAP2 of the patients in the two groups was negative correlation with their expression of miR-484(r=-0.498)(all P<0.05).The expressions of MAP2 and miR-484 in ovarian malignant cysts tissues of the patients were not related to their age,pathological type,tumor diameter and degree of invasion,but were related to their degree of differentiation,lymph node metastasis,omental metastasis,FIGO stage,and histopathological grade(all P<0.05).Kaplan-Meier analysis showed that the 3-year cumulative survival rate(44.9%)of the patients with high MAP2 expression was significantly lower than that(65.8%)of the patients with low MAP2 expression(Log rankχ^(2)=7.432,P=0.006).The 3-year cumulative survival rate(65.8%)of the patients with high miR-484 expression was significantly higher than that(44.0%)of the patients with low miR-484 expression(Log rankχ^(2)=8.819,P=0.003).Cox results showed that the high expression of MAP2,the low expression of miR-484,the lymph node metastasis,the FIGO stage,the degree of differentiation,the high expression of MAP2,the low expression of miR-484,and the omentum metastasis of the patients were all the risk factors affecting their prognosis.Conclusion:The expression of miR-484 in ovarian malignant cyst tissues of the patients is low and the expression of MAP2 is high.The expression of miR-484 and the expression of MAP2 are negatively correlated each other,and both of which are closely related to the development and prognosis of ovarian malignant cysts of the patients.