鞘氨醇单胞菌的特征特性及对石油成分降解作用的分析预测

Characteristics of Sphingomonas and Prediction of Its Degrading Effect on Petroleum Components

石油在我国经济社会发展中具有重要作用,但目前我国尚缺乏微生物对油品污染的研究。鞘氨醇单胞菌属(Sphingomonas)中的多个种因降解多环芳烃的能力而日益受到关注。本研究通过实验证明了鞘氨醇单胞菌对多环芳烃菲较强的降解能力,并通过UniProt数据库对鞘氨醇单胞菌是否存在一般烷烃降解酶类进行检索。同时,重点了解多噬香鞘氨醇单胞菌与邻苯二酚-2,3-双加氧酶的基本特性,运用生物信息学方法预测分析其氨基酸理化性质、同源性、蛋白质二级和三级结构以及KEGG通路等。结果表明,矢野氏鞘氨醇单胞菌等鞘氨醇单胞菌属细菌可能具有降解甲烷及多碳烷烃的能力。由于与多噬香鞘氨醇单胞菌邻苯二酚-2,3-双加氧酶基因同源性较高,矢野氏鞘氨醇单胞菌很可能同样具有多环芳烃降解能力,分析结果将通过试验进一步验证,并为后续研究做好准备。

Petroleum plays an indispensable role in the economic and social development of China,but there is still a lack of the research on the role of microorganisms in oil pollution.Many species of bacteria of Sphingomonas have attracted increasing attention for their ability to degrade polycyclic aromatic hydrocarbons(PAHs).In this study,it was proved that Sphingomonas had a strong degrading effect on PAHs of phenanthrene through experiments,and the existence of general alkane-degrading enzymes in Sphingomonas polyphages was search by UniProt database.At the same time,we focused on the basic characteristics of Sphingomonas polyphages and catechol 2,3-dioxygenase,and used the bioinformatics methods to predict and analyze their amino acid physical and chemical properties,homology,protein secondary and tertiary structure,KEGG pathway,etc.The results showed that Sphingomonas yanoikuyae and other bacteria of the genus Sphingomonas may have the ability to degrade both methane and polycarbon alkanes.Due to the high homology with catechol 2,3-dioxygenase gene of Sphingomonas polyphages,Sphingomonas sagittarius may also have the ability to degrade polycyclic aromatic hydrocarbons.The analysis results will be further verified by experiments and prepared for subsequent studies.