无花果乳浆的化学组分分析、质量标准建立及对A549细胞的影响

Analysis of chemical components, establishment of quality standard of fig fruit latex and its effect on A549 cells

目的:定性、定量考察无花果乳浆(FFL)中的主要化学组分,初步建立质量标准,并考察其体外抗肿瘤活性。方法:采用薄层色谱法和高效液相色谱法定性和定量检测FFL中补骨脂素和佛手柑内酯的质量分数(m∶m),采用紫外-可见分光光度法检测总多糖和总蛋白质的含量,并检查水分、总灰分和酸不溶性灰分的含量,以建立质量标准。MTT法、细胞划痕实验考察长波长紫外线(UVA)对FFL光化学活性影响。结果:5个批次的FFL含水量、总灰分、酸不溶性灰分、总多糖、总蛋白、补骨脂素和佛手柑内酯的平均含量分别为81.57%、3.65%、0.079%、0.568%、3.77%、0.0604%、0.0192%,RSD分别为0.010%、0.108%、0.111%、0.122%、0.134%、0.014%、0.149%。FFL经UVA辐射后具有光化学活性,可抑制A549细胞的增殖和迁移。结论:本研究建立的成分检验方法简便、快速、重复性好,适用于FFL的组分研究与质量控制,FFL在体外具有光化学活性及抗A549肺癌活性。

Objective: To establish a preliminary quality standard, qualitatively and quantitatively analyze the main chemical components of fig fruit latex(FFL), as well as its anti-tumor efficacy in vitro. Methods: Psoralen and bergapten(m :m) were determined qualitatively and quantitatively in FFL using thin-layer chromatography(TLC) and high performance liquid chromatography(HPLC). To establish the quality standard, total polysaccharide and total protein levels, as well as the amounts of water, total ash, and acid insoluble ash, were measured using ultraviolet-visible spectrophotometry. MTT and the cell scratch test were used to examine how long wavelength ultraviolet(UVA) affected the photochemical activity of FFL. Results: The average concentrations of psoralen, bergapten, total polysaccharide, total protein, total ash, total ash, acid-insoluble ash, and water in 5 batches of FFL were 81.57%, 3.65%, 0.079%, 0.568%, 3.77%, 0.0604% and 0.0192%, respectively. RSD were, respectively,0.010%, 0.108%, 0.111%, 0.122%, 0.134%, 0.014% and 0.149%. Following UVA exposure, FFL showed photochemical activity that may reduce the proliferation and migration of A549 cells. Conclusion: The method established in this study was straightforward, quick, and repeatable, making it appropriate for the composition analysis and FFL quality control. In vitro, FFL possessed anti-lung cancer A549 action and photochemical activity.