不同水体沉水植物叶面微生物群落特征

Characteristics of microbial community on leaf surface of submerged plants in different water bodies

以沉水植物黑藻(Hydrilla verticillata)、苦草(Vallisneria natans)、伊乐藻(Elodea nuttallii)和菹草(Potamogeton crispus L)为研究对象,利用扫描电镜、荧光显微镜和16S rRNA基因高通量测序技术,探究不同生境中沉水植物叶面附着微生物群落结构及影响因素。结果表明:沉水植物叶面附着藻类组成与环境水体的藻类组成具有明显差异;不同沉水植物叶面附着微生物密度不同,且河流样品中高于湖泊样品;α-变形菌、β-变形菌和γ-变形菌纲是沉水植物叶面附着生物膜上的主要细菌纲,这些纲具有降解污染物质、净化水体的功能;水体营养盐浓度对沉水植物叶面附着微生物群落结构有很大影响。

Submerged plants, such as the Hydrilla verticillata, Vallisneria natans, Elodea nuttallii and Potamogeton crispus L, were selected as the research objects. This study used the scanning electron microscopy, fluorescence microscopy and 16S rRNA high-throughput sequencing technology to explore the bacterial community structure and influencing factors attached to the leaf surface of submerged plants in different habitats. The results show that the composition of algae attached to the leaf surface of submerged plants is significantly different from that in the environmental water body. Different submerged plants have different density of microorganisms on the leaf surface, and the density of microorganisms attached to the surface of lake samples are lower than the river samples. α-Proteobacteria, β-Proteobacteria and γ-Proteobacteria are the main bacterial classes in the biofilm on the leaves of submerged plants. These classes have the function of degrading pollutants and purifying water bodies. Redundant analysis shows that the concentration of nutrients in the water body has a great influence on the microbial community structure on the leaf surface of submerged plants.