牛肝素脱色工艺研究

Decolorization Process of Bovine Heparin

将牛源粗品肝素经脱蛋白处理后,分别采用双氧水工艺、过氧乙酸工艺、高锰酸钾工艺等3种氧化工艺精制牛肝素,研究了不同氧化工艺对牛肝素脱色效果和结构的影响。结果表明,双氧水工艺、过氧乙酸工艺、高锰酸钾工艺的脱色率分别为92.0%、96.0%、95.9%,回收率分别为96.0%、91.3%、95.3%;经双氧水氧化后,牛肝素的双糖结构变化不大;经过氧乙酸氧化后,ΔGlyser被氧化为ΔGlyser ox1;经高锰酸钾氧化后,ΔGlyser被氧化为ΔGlyser ox1和ΔGlyser ox2;将精制的牛肝素制成注射液后,双氧水工艺牛肝素注射液的颜色变深速度最快,其次依次为过氧乙酸工艺、高锰酸钾工艺。综合评价,高锰酸钾工艺为牛肝素的最佳脱色工艺。

Firstly,we deproteinized crude bovine heparin,and then refined bovine heparin by three oxidation processes,including hydrogen peroxide process,peracetic acid process,and potassium permanganate process.Moreover,we studied the effects of different oxidation processes on the decolorization and structure of bovine heparin.The results show that the decolorization rates of hydrogen peroxide process,peracetic acid process,and potassium permanganate process are 92.0%,96.0%,and 95.9%,and the recoveries are 96.0%,91.3%,and 95.3%,respectively.After oxidation with hydrogen peroxide,the disaccharide structure of bovine heparin negligibly changes.After peracetic acid oxidation,ΔGlyser is oxidized toΔGlyser ox1.After potassium permanganate oxidation,ΔGlyser is oxidized toΔGlyser ox1 andΔGlyser ox2.After bovine heparin is made into injection,the color darkening rate of bovine heparin injection refined by hydrogen peroxide process is the fastest,followed by peracetic acid process and potassium permanganate process.The comprehensive evaluation indicates that potassium permanganate process is the best decolorization process of bovine heparin.