红角辉蝽和紫翅果蝽线粒体完整基因组测序及蝽亚科系统发育分析(半翅目: 蝽科)

Sequencing of the complete mitochondrial genomes of Carbula crassiventris and Carpocoris purpureipennis and phylogenetic analysis of Pentatominae (Hemiptera: Pentatomidae)

【目的】本研究对红角辉蝽Carbula crassiventris和紫翅果蝽Carpocoris purpureipennis完整线粒体基因组测序,以探究蝽亚科(Pentatominae)线粒体基因组特征并重建其系统发育关系。【方法】使用Illumina MiSeq测序平台测定红角辉蝽和紫翅果蝽线粒体基因组全序列,并进行组装和注释。基于这2个种和其他30个蝽亚科分类单元线粒体基因组的13个蛋白质编码基因的第1和2位密码子以及2个rRNA基因的核苷酸序列,利用贝叶斯和最大似然法重建蝽亚科系统发育树。【结果】红角辉蝽和紫翅果蝽的线粒体基因组全长分别为15824和16575 bp,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。蝽亚科内线粒体基因组基因排列顺序保守且没有发现基因重排。此外,蝽亚科内的碱基组成、密码子使用和RNA结构均较为保守;控制区重复序列拥有不同的长度、类型和拷贝数。基于贝叶斯法和最大似然法重建的系统发育树显示二星蝽族(Eysarcorini)、果蝽族(Carpocorini)、稻绿蝽族(Nezarini)和Antestiini构成一个稳定分枝。【结论】系统发育分析支持辉蝽属Carbula应属于二星蝽族,而果蝽属Carpocoris、斑须蝽属Dolycoris和珠蝽属Rubiconia同属于果蝽族。

【Aim】In this study,the complete mitochondrial genomes(mtgenomes)of Carbula crassiventris and Carpocoris purpureipennis were sequenced to investigate the mitogenomic characteristics and reconstruct the phylogenetic relationships of Pentatominae.【Methods】The complete mtgenomes of Carbula crassiventris and Carpocoris purpureipennis were sequenced using the Illumina MiSeq Platform,and the resulted sequences were assembled and annotated.The phylogenetic trees of Pentatominae were reconstructed using Bayesian inference(BI)and maximum likelihood(ML)methods based on the sequences of the 1st and 2nd positions of the codons of the 13 proteincoding genes(PCGs)and the nucleotide sequences of the two rRNA genes of the mtgenome of the two species and other 30 Pentatominae taxa.【Results】The mtgenome of Carbula crassiventris and Carpocoris purpureipennis are 15824 and 16575 bp in length,respectively,with 13 PCGs,2 rRNA genes,22 tRNA genes,and a control region.The mtgenome gene arrangements are conserved within Pentatominae and no rearrangement was detected.In addition,the base composition,codon usage and RNA structures are conserved within Pentatominae.The length,types and copies of repeat units in control regions are different among species.The phylogenetic tree based on BI and ML showed a stable clade comprised of Eysarcorini,Carpocorini,Nezarini and Antestiini.【Conclusion】The phylogenetic analysis confirmed that Carbula belongs to Eysarcorini,and Carpocoris,Dolycoris and Rubiconia belong to Carpocorini.