摘要
CRISPR-Cas9,-Cas12a,-Cas12b,and-Cas13 have been harnessed for genome engineering in human and plant cells(Liu et al.,2022).However,the large size of these Cas proteins(e.g.190 kDa for SpCas9)makes them difficult to deliver into cells via a viral vector.The development of smaller Cas proteins will lead to reduced viral vector sizes that can be more widely adopted in versatile genome engineering systems.Recently,a CRISPR-Cas12j2(CasF)system was discovered in huge phages and developed into a hypercompact genome editor due to the small size of Cas12j2(80 kDa)(Pausch et al.,2020).Unfortunately,the gene editing efficiency of Cas12j2 in Arabidopsis protoplasts using ribonucleoprotein delivery was less than one percent(Pausch et al.,2020).Further optimization of this system is clearly required if CRISPR-Cas12j2-mediated editing in plant genomes is to be adopted by the plant sciences community.
基金
supported by the National Key Research and Development Program of China(award no.NK2022010204)to Y.Z.
the National Natural Science Foundation of China(award nos.32270433,32101205,32072045,and 31960423)to X.T.,X.Z.,and Y.Z.
the Sichuan Science and Technology Program(award no.2021JDRC0032)to Y.Z.
the Technology Innovation and Application Development Program of Chongqing(award no.CSTC2021JSCX-CYLHX0001)to X.T.and Y.Z.
supported by the National Science Foundation Plant Genome Research Program grant(award nos.IOS-1758745 and IOS2029889)
USDA-AFRI Agricultural Innovations Through Gene Editing Program(award no.2021-67013-34554)to Y.Q.S.S.is a fellow of the Foundation for Food and Agriculture Research.
