甲基磺酸乙酯处理对紫丁香种子萌发及幼苗表型的影响

Effects of Ethyl Methyl Sulfone Mutation on Germination and Phenotypic Variation of Syringa oblata

通过甲基磺酸乙酯(EMS)诱变筛选获得性状优良的紫丁香(Syringa oblata)新种质,为后续育种以及开展功能基因组的相关研究提供适宜的种质资源。本研究利用EMS处理紫丁香种子,设4个浓度梯度(0%、0.1%、0.2%、0.3%),以及3个处理时间(24、48、72 h),分析不同处理对种子萌发和成苗的影响,得到适宜的处理参数,进一步分析变异植株的表型变异特征。EMS处理可显著降低紫丁香种子萌发率和成苗率(P<0.05);且随着EMS浓度与处理时间的增加,苗高也受到不同程度抑制;当0.3%EMS处理干种子48 h和0.2%EMS处理湿种子72 h时,两种种子状态的萌发率和成苗率降为对照的50%。经过1年观测,紫丁香共获得叶形、叶色和株形3种表现稳定的变异类型,变异率为1.28%,0.2%处理干种48 h变异率为0.51%,为EMS诱变紫丁香种子的最佳参数。

To obtain the basic materials of Syringa oblata varieties with good characters by mutagenesis and screening with ethyl methane sulfonate(EMS),so as to provide favorable germplasm resources for subsequent breeding and functional genome research.In this study,the seeds of Syringa oblata were treated with EMS.Four concentration gradients(0%,0.1%,0.2% and 0.3%)and three treatment times(24 h,48 h and 72 h)were set to analyze the germination rate and seedling rate,thus obtaining appropriate treatment parameters to further analyze the phenotypic characteristics of variant plants.EMS treatment could significantly reduce the germination and seedling rate of Syringa oblata seeds(P<0.05).With the increase of EMS concentration and treatment time,the seedling height was also inhibited to varying degrees;When 0.3% EMS was used to treat dry seeds for 48 hours and 0.2% EMS was applied to treat wet seeds for 72 hours,the germination rate and seedling rate of the two seed states decreased to 50% of the control.After one year’s observation,from Syringa oblata,three stable variation types of leaf shape were obtained,leaf color and plant shape,with a mutation rate of 1.28%.The 48 h variation rate of dry seeds treated with 0.2% was 0.51%,which was the best parameter for EMS mutagenesis of Syringa oblata seeds.