不同玉竹种质资源多糖含量检测及ISSR分析

Determination and ISSR Analysis on Polysaccharide Content in Different Germplasm Resources of Polygonatum odoratum

为了阐明不同玉竹种质资源的多糖含量差异及遗传相似性特点,采用紫外-可见光光度法检测17个玉竹种质资源多糖含量,通过ISSR分子标记法对供试玉竹的遗传结构进行分析。结果表明,17个玉竹种质资源多糖含量在6.12%~9.33%之间,17个玉竹种质资源的多糖含量之间呈显著性差异,在1%显著水平下,除1号和5号外,其余均存在显著性差异。17个玉竹种质资源的遗传相似性范围在0.1638~0.9230之间,通过构建系统发育树,在遗传相似系数为0.56处将17个玉竹分为三类。研究表明,玉竹种质资源之间的亲缘性与地理分布呈现一定的相关性,玉竹多糖含量与地理来源没有明显的关系。

To clarify the difference of polysaccharide content and genetic similarity of different Polygonatum odoratum germplasm resources,the polysaccharide content of 17 Polygonatum odoratum germplasm resources was detected by UV-vis spectrophotometry,and the genetic structure of Polygonatum odoratum was analyzed by ISSR molecular marker method.The results showed that the polysaccharide content of 17 Polygonatum odoratum germplasm resources was from 6.12%to 9.33%.There were significant differences in the polysaccharide content of 17 Polygonatum odoratum germplasm resources.At the 1%significant level,there were significant differences in all except No.1 and No.5.The genetic similarity of 17 germplasm resources of Polygonatum odoratum ranged from 0.1638 to 0.9230.By constructing phylogenetic tree,17 Polygonatum odoratum were divided into three categories at the genetic similarity coefficient of 0.56.The study made it clear that there was a certain correlation between the genetic relationship and geographical distribution of Polygonatum odoratum germplasm resources,and there was no obvious relationship between Polygonatum odoratum polysaccharide content and geographical source.